Q.L. Genghis Harris1, Stephen J. Lewis2, Nicole A. Young2, Frank Vajda2, Bevyn Jarrott2
1University of Melbourne, Austin General Hospital, Heidelberg, Victoria, Australia.
2University of Melbourne, Clinical Pharmacology and Therapeutics Unit, Austin and Repatriation General Hospitals, Heidelberg, Victoria, Australia
Tóm tắt
SUMMARY1. The possibility that the anticonvulsant activity of the benzodiazepines, diazepam and clobazam, is related to changes in EEG parameters, particularly β activity, was investigated in amygdaloid kindled rats.2. The effects of diazepam (1, 2, 4, 8 and 16 μmol/kg), administered intraperitoneally (i.p.), clobazam (1, 2, 4, 8, 16 and 32 μmol/kg, i.p.) or vehicle (dimethyl sulfoxide) on the cortical EEG of amygdaloid kindled rats were quantitated for 15 min using computerized period amplitude analysis. Immediately afterwards, the amygdala was stimulated and the after‐discharge duration (AD) and the seizures stage (SS) were determined.3. The equivalent percentage time (EPT) of the diazepam‐treated group was decreased in the θ band (4–8 Hz) and increased in the α (8–12 Hz) and first six β (12–36 Hz) bands. The mean peak amplitude (MPA) was increased in the α (8–12 Hz) and all seven β bands (12–40 Hz). Clobazam increased the EPT and MPA in the α (8–12 Hz) and all seven β (12–40 Hz) bands. The MPA was also increased by clobazam in the θ (4–8 Hz) band.4. Diazepam reduced both the AD and SS of the kindled seizures at doses of 4, 8 and 16 μmol/kg, whereas clobazam was anticonvulsant at doses of 16 and 32 μmol/kg. The reduction in both AD and SS correlated with increases in the EPT and MPA in the first β (12–16 Hz) band in the diazepam‐treated group and in the first four β (12–28 Hz) bands in the clobazam‐treated group.
