Quantitative analysis of peripheral blood Th0, Th1, Th2 and the Th1:Th2 cell ratio during normal human pregnancy and preeclampsia

Clinical and Experimental Immunology - Tập 117 Số 3 - Trang 550-555 - 2001
Shigeru Saito1, Masatoshi Sakai1, Yasushi Sasaki1, Kyoko Tanebe1, Hiroshi Tsuda1, T Michimata1
1Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Toyama, Japan

Tóm tắt

SUMMARYWe calculated the percentage of Th1, Th2, Th0 cells and the Th1:Th2 cell ratio of peripheral blood from normal pregnant subjects and preeclampsia patients using flow cytometry which can analyse both the surface marker, CD4, and intracellular cytokines, interleukin (IL)-4 and interferon (IFN)-γ. In normal pregnancy, the percentage of Th1 cells was significantly lower in the third trimester, and the ratios of Th1:Th2 were significantly lower in the second and third trimester than in nonpregnant subjects. In contrast, the percentage of Th1 cells and the ratios of Th1:Th2 in preeclampsia were significantly higher than in normal third trimester pregnant subjects. The percentage of Th2 cells in preeclampsia was significantly lower than in third trimester of normal pregnancy. Additionally, peripheral blood mononuclear cells from these subjects and patients were cultured with phytohemagglutinin stimulation, and IL-4 and IFN-γ concentrations were determined in the supernatant by enzymed linked immunosorbent assays. The percentage of Th1 and Th2, and the ratios of Th1:Th2 were correlated with cytokine (IFN-γ and IL-4) secretion level. These results demonstrated that Th2 cells were predominant in the second and third trimesters of normal pregnancy, but Th1 cells predominated in preeclamptic patients.

Từ khóa


Tài liệu tham khảo

10.1016/0167-5699(96)80606-2

10.1016/0167-5699(93)90235-D

Lin H, 1993, J Immunol, 151, 4562, 10.4049/jimmunol.151.9.4562

Delassus S, 1994, J Immunol, 152, 2411, 10.4049/jimmunol.152.5.2411

Chaouat G, 1995, J Immunol, 154, 4261, 10.4049/jimmunol.154.9.4261

Krishnan L, 1996, J Immunol, 156, 644, 10.4049/jimmunol.156.2.644

Krishnan L, 1996, J Immunol, 156, 653, 10.4049/jimmunol.156.2.653

10.1046/j.1365-2249.1996.d01-809.x

Matthiesen L, 1998, Am J Reprod Immunol, 39, 362, 10.1111/j.1600-0897.1998.tb00370.x

Russell AS, 1997, J Rheumatol, 24, 1045

Ekerfelt C, 1997, Am J Reprod Immunol, 38, 320, 10.1111/j.1600-0897.1997.tb00307.x

Sunder-Plassmann G, 1989, J Autoimmun, 2, 203, 10.1016/0896-8411(89)90156-X

Hamai Y, 1997, Am J Reprod Immunol, 38, 89, 10.1111/j.1600-0897.1997.tb00281.x

10.1016/0165-0378(96)00976-X

Daniel Y, 1998, Am J Reprod Immunol, 39, 376, 10.1111/j.1600-0897.1998.tb00372.x

Omu AE, 1995, Nutrition, 11, 688

Vince GS, 1995, Br J Obstet Gynecol, 102, 20, 10.1111/j.1471-0528.1995.tb09020.x

Greer IA, 1994, Obstet Gynecol, 84, 937

10.1016/S0165-0378(97)00028-4

Saito S, 1999, Am J Reprod Immunol, 41, 297, 10.1111/j.1600-0897.1999.tb00442.x

10.1001/jama.273.24.1933

10.1038/2006

10.1084/jem.169.2.569

Biassoni R, 1991, Scand J Immunol, 33, 247, 10.1111/j.1365-3083.1991.tb01769.x

10.1146/annurev.immunol.6.1.485

10.1016/0022-1759(95)00209-X

10.1038/373255a0

Testi R, 1989, J Immunol, 142, 1854, 10.4049/jimmunol.142.6.1854

29 S Saito, N Tsukaguchi, T Hasegawa, T Michimata, H Tsuda, and N Narita . Distribution of Th1, Th2 and Th0 and the Th1/Th2 cell ratios in human peripheral and endometrial T cells . Am J Reprod Immunol1999 ; in press.

Piccini MP, 1995, J Immunol, 155, 128, 10.4049/jimmunol.155.1.128

Manetti R, 1994, J Exp Med, 13, 1273, 10.1084/jem.179.4.1273

Zhou Y, 1993, J Clin Invest, 91, 950, 10.1172/JCI116316

Meekins JW, 1994, Br J Obstet Gynecol, 101, 669, 10.1111/j.1471-0528.1994.tb13182.x

10.1016/0008-8749(90)90219-H

10.1006/cimm.1993.1316

Thông tin
Thông tin xuất bản

Clinical and Experimental Immunology

Tập 117 Số 3

550-555

Thông tin tác giả