Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray

American Association for the Advancement of Science (AAAS) - Tập 270 Số 5235 - Trang 467-470 - 1995
Mark Schena1, Dari Shalon1, Ronald W. Davis1, Patrick O. Brown1
1M. Schena and R. W. Davis, Department of Biochemistry, Beckman Center, Stanford University Medical Center, Stanford, CA 94305, USA.

Tóm tắt

A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.

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Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 270 Số 5235

467-470

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