Quantitative Analysis of Mycobacterial and Propionibacterial DNA in Lymph Nodes of Japanese and European Patients with Sarcoidosis

Journal of Clinical Microbiology - Tập 40 Số 1 - Trang 198-204 - 2002
Yoshinobu Eishi1, Moritaka Suga2, Ikuo Ishige1, Daisuke Kobayashi1, Tetsuo Yamada1, Tamiko Takemura3, Touichiro Takizawa1, Morio Koike1, Shoji Kudoh4, Ulrich Costabel5, Josune Guzman6, G Rizzato7, Gabriella Fontanini8, Ronald du Bois9, Andrew G. Nicholson10, Om P. Sharma11, Masayuki Ando2
1Department of Human Pathology, School of Medicine, Tokyo Medical and Dental University
2First Department of Internal Medicine, School of Medicine, Kumamoto University, Kumamoto
3Department of Pathology, Japanese Red Cross Medical Center
4Fourth Department of Internal Medicine, Nippon Medical School
5Department of Pneumology and Allergology, Ruhrlandklinik, Essen
6Department of General and Experimental Pathology, Bochum University, Bochum, Germany
7Sarcoidosis Clinic
8Institute of Pathology, Niguarda Hospital, Milan, Italy
9Interstitial Lung Disease Unit
10Department of Histopathology, Royal Brompton Hospital, London, England
11Department of Medicine, School of Medicine, University of Southern California, Los Angeles, California 90033

Tóm tắt

ABSTRACTThe cause(s) of sarcoidosis is unknown.Mycobacteriumspp. are suspected in Europe andPropionibacteriumspp. are suspected in Japan. The present international collaboration evaluated the possible etiological links between sarcoidosis and the suspected bacterial species. Formalin-fixed and paraffin-embedded sections of biopsy samples of lymph nodes, one from each of 108 patients with sarcoidosis and 65 patients with tuberculosis, together with 86 control samples, were collected from two institutes in Japan and three institutes in Italy, Germany, and England. Genomes ofPropionibacterium acnes,Propionibacterium granulosum,Mycobacterium tuberculosis,Mycobacterium aviumsubsp.paratuberculosis, andEscherichia coli(as the control) were counted by quantitative real-time PCR. EitherP. acnesorP. granulosumwas found in all but two of the sarcoid samples.M. aviumsubsp.paratuberculosiswas found in no sarcoid sample.M. tuberculosiswas found in 0 to 9% of the sarcoid samples but in 65 to 100% of the tuberculosis samples. In sarcoid lymph nodes, the total numbers of genomes ofP. acnesorP. granulosumwere far more than those ofM. tuberculosis. P. acnesorP. granulosumwas found in 0 to 60% of the tuberculosis and control samples, but the total numbers of genomes ofP. acnesorP. granulosumin such samples were less than those in sarcoid samples.Propionibacteriumspp. are more likely thanMycobacteriaspp. to be involved in the etiology of sarcoidosis, not only in Japanese but also in European patients with sarcoidosis.

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Journal of Clinical Microbiology

Tập 40 Số 1

198-204

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