Quantitation of Hepatic Glycogenolysis And Gluconeogenesis in Fasting Humans With 13 C NMR

American Association for the Advancement of Science (AAAS) - Tập 254 Số 5031 - Trang 573-576 - 1991
Douglas L. Rothman1, I Magnusson1, Lee D. Katz2, Robert G. Shulman3, Gerald I. Shulman4
1Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510.
2Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, CT 06510.
3Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06510.
4Departments of Internal Medicine and Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CIT 06510.

Tóm tắt

The rate of net hepatic glycogenolysis was assessed in humans by serially measuring hepatic glycogen concentration at 3- to 12-hour intervals during a 68-hour fast with 13 C nuclear magnetic resonance spectroscopy. The net rate of gluconeogenesis was calculated by subtracting the rate of net hepatic glycogenolysis from the rate of glucose production in the whole body measured with tritiated glucose. Gluconeogenesis accounted for 64 ± 5% (mean ± standard error of the mean) of total glucose production during the first 22 hours of fasting. In the subsequent 14-hour and 18-hour periods of the fast, gluconeogenesis accounted for 82 ± 5% and 96 ± 1% of total glucose production, respectively. These data show that gluconeogenesis accounts for a substantial fraction of total glucose production even during the first 22 hours of a fast in humans.

Từ khóa


Tài liệu tham khảo

ABUMRAD, N.N., ABSORPTION AND DISPOSITION OF A GLUCOSE-LOAD IN THE CONSCIOUS DOG, AMERICAN JOURNAL OF PHYSIOLOGY 242: E398 (1982).

ADKINS, B.A., IMPORTANCE OF THE ROUTE OF INTRAVENOUS GLUCOSE DELIVERY TO HEPATIC GLUCOSE BALANCE IN THE CONSCIOUS DOG, JOURNAL OF CLINICAL INVESTIGATION 79: 557 (1987).

BJOKMAN, O, DIABETES 39: 747 (1990).

BJORKMAN, O, ROLE OF THE KIDNEY IN THE METABOLISM OF FRUCTOSE IN 60-HOUR FASTED HUMANS, DIABETES 31: 516 (1982).

BOTTOMLEY, P.A., PROTON-DECOUPLED, OVERHAUSER-ENHANCED, SPATIALLY LOCALIZED C-13 SPECTROSCOPY IN HUMANS, MAGNETIC RESONANCE IN MEDICINE 12: 348 (1989).

Cherrington, A.D., Hormonal Control of Gluconeogenesis: 15 (1986).

CONSOLI, A, JOURNAL OF CLINICAL INVESTIGATION 80: 1301 (1987).

GRUETTER, R, C-13 NMR VISIBILITY OF RABBIT MUSCLE GLYCOGEN INVIVO, MAGNETIC RESONANCE IN MEDICINE 20: 327 (1991).

HAASE, A, FLASH IMAGING - RAPID NMR IMAGING USING LOW FLIP-ANGLE PULSES, JOURNAL OF MAGNETIC RESONANCE 67: 258 (1986).

HETENYI, G, CORRECTION FOR METABOLIC EXCHANGE IN THE CALCULATION OF THE RATE OF GLUCONEOGENESIS IN RATS, BIOCHEMICAL MEDICINE 29: 372 (1983).

Hetenyi, G., Hormonal Control of Gluconeogenesis: 79 (1986).

Hull, W. E., Abstracts of the Sixth Society of Magnetic Resonance in Medicine Meeting: 488 (1987).

JACKSON, C.L., C-13 NMR OF GLYCOGEN - HYDRATION RESPONSE STUDIED BY USING SOLIDS METHODS, BIOCHEMISTRY 28: 5024 (1989).

JUE, T, DIRECT OBSERVATION OF GLYCOGEN-SYNTHESIS IN HUMAN-MUSCLE WITH C-13 NMR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 4489 (1989).

KATZ, J, DETERMINATION OF GLUCONEOGENESIS INVIVO WITH C-14-LABELED SUBSTRATES, AMERICAN JOURNAL OF PHYSIOLOGY 248: R391 (1985).

KREBS, H.A., RENAL GLUCONEOGENESIS, ADVANCES IN ENZYME REGULATION 1: 385 (1963).

KREBS, H.A., FATE OF ISOTOPIC CARBON IN KIDNEY CORTEX SYNTHEZING GLUCOSE FROM LACTATE, BIOCHEMICAL JOURNAL 101: 242 (1966).

KREISBERG, R.A., ALANINE AND GLUCONEOGENESIS IN MAN - EFFECT OF ETHANOL, JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM 34: 876 (1972).

MAGNUSSON, I, QUANTITATION OF THE PATHWAYS OF HEPATIC GLYCOGEN FORMATION ON INGESTING A GLUCOSE-LOAD, JOURNAL OF CLINICAL INVESTIGATION 80: 1748 (1987).

MCGARRY, J.D., JOURNAL OF CLINICAL INVESTIGATION 74: 1901 (1984).

NILSSON, L.H., LIVER-GLYCOGEN IN MAN - EFFECT OF TOTAL STARVATION OR A CARBOHYDRATE-POOR DIET FOLLOWED BY CARBOHYDRATE REFEEDING, SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION 32: 325 (1973).

ORDIDGE, R.J., IMAGE-SELECTED INVIVO SPECTROSCOPY (ISIS) - A NEW TECHNIQUE FOR SPATIALLY SELECTIVE NMR-SPECTROSCOPY, JOURNAL OF MAGNETIC RESONANCE 66: 283 (1986).

OWEN, O.E., LIVER AND KIDNEY METABOLISM DURING PROLONGED STARVATION, JOURNAL OF CLINICAL INVESTIGATION 48: 574 (1969).

SCHUMANN, W.C., METABOLISM OF [2-C-14]ACETATE AND ITS USE IN ASSESSING HEPATIC KREBS CYCLE ACTIVITY AND GLUCONEOGENESIS, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 6985 (1991).

SHALWITZ, R.A., VISIBILITY OF MAMMALIAN HEPATIC GLYCOGEN TO THE NMR EXPERIMENT, INVIVO, MAGNETIC RESONANCE IN MEDICINE 5: 462 (1987).

SHULMAN, G.I., QUANTITATIVE COMPARISON OF PATHWAYS OF HEPATIC GLYCOGEN REPLETION IN FED AND FASTED HUMANS, AMERICAN JOURNAL OF PHYSIOLOGY 259: E335 (1990).

SHULMAN, G.I., C-13 NMR-STUDIES OF GLYCOGEN TURNOVER IN THE PERFUSED RAT-LIVER, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 5027 (1988).

SILLERUD, L.O., BIOCHEMISTRY-US 22: 1087 (1983).

SILVER, M.S., SELECTIVE-POPULATION INVERSION IN NMR, NATURE 310: 681 (1984).

STRISOWER, E.H., INCORPORATION OF ACETATE CARBON INTO GLUCOSE BY LIVER SLICES FROM NORMAL AND ALLOXAN-DIABETIC RATS, JOURNAL OF BIOLOGICAL CHEMISTRY 198: 115 (1952).

TAYLOR R unpublished data.

WAHREN, J, SPLANCHNIC AND PERIPHERAL GLUCOSE AND AMINO-ACID METABOLISM IN DIABETES-MELLITUS, JOURNAL OF CLINICAL INVESTIGATION 51: 1870 (1972).

WEINMAN, E, PHYSIOL REV 37: 252 (1952).

ZANG, L.H., C-13 NMR RELAXATION-TIMES OF HEPATIC GLYCOGEN INVITRO AND INVIVO, BIOCHEMISTRY 29: 6815 (1990).

Thông tin
Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 254 Số 5031

573-576

Thông tin tác giả