Quantifying the dynamics of light tolerance in<scp><i>A</i></scp><i>rabidopsis</i>plants during ontogenesis

Plant, Cell and Environment - Tập 38 Số 12 - Trang 2603-2617 - 2015
Fabrício E. L. Carvalho1, Maxwell A. Ware1, Alexander V. Ruban1
1School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, UK

Tóm tắt

AbstractThe amount of light plants can tolerate during different phases of ontogenesis remains largely unknown. This was addressed here employing a novel methodology that uses the coefficient of photochemical quenching (qP) to assess the intactness of photosystemIIreaction centres. Fluorescence quenching coefficients, total chlorophyll content and concentration of anthocyanins were determined weekly during the juvenile, adult, reproductive and senescent phases of plant ontogenesis. This enabled quantification of the protective effectiveness of non‐photochemical fluorescence quenching (NPQ) and determination of light tolerance. The light intensity that caused photoinhibition in 50% of leaf population increased from ∼70 μmol m−2 s−1, for 1‐week‐old seedlings, to a maximum of 1385 μmol m−2 s−1for 8‐week‐old plants. After 8 weeks, the tolerated light intensity started to gradually decline, becoming only 332 μmol m−2 s−1for 13‐week‐old plants. The dependency of light tolerance on plant age was well‐related to the amplitude of protectiveNPQ(pNPQ) and the electron transport rates (ETRs). Light tolerance did not, however, show a similar trend to chlorophylla/bratios and content of anthocyanins. Our data suggest that pNPQis crucial in defining the capability of high light tolerance byArabidopsisplants during ontogenesis.

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Plant, Cell and Environment

Tập 38 Số 12

2603-2617

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