Quantification of Choline-containing Compounds in Malignant Breast Tumors by 1H MR Spectroscopy Using Water as an Internal Reference at 1.5 T

Magnetic Resonance Materials in Physics, Biology and Medicine - 2006
Hyun-Man Baik1, Min-Ying Su1, Hon Yu1, Orhan Nalcioglu1, Rita Mehta2
1John Tu and Thomas Center for Functional Onco-Imaging, University of Califorinia, Irvine, USA
2Department of Medicine, University of California, Irvine, USA

Tóm tắt

The quantification of choline-containing compounds (Cho) in breast tumors by proton MR spectroscopy (1H-MRS) has been of great interest because such compounds have been linked to malignancy. In this study, an internal reference method for the absolute quantification of Cho metabolite in malignant breast tumors was presented using a clinical 1.5 T scanner. We performed in vitro measurements to examine the accuracy of absolute quantification using four phantoms of known choline chloride concentrations. There was a high correlation between the calculated concentrations by MRS and the known concentrations (r 2 > 0.98). We applied the technique to in vivo breast study conducted on 45 patients with biopsy-confirmed breast cancer. After T 1 and T 2 relaxation times were corrected, the Cho levels in this work had a range of 0.76 – 21.20 mmol/kg from 34 MR spectra of 32 patients with malignant breast lesions. This result was rather consistent with the previously published value (i.e., 1.38 – 10 mmol/kg, Bolan et al. in Magn Reson Med 50:1134–1143, 2003). Therefore, we conclude that the internal method using the fully relaxed water as a reference could be used for quantifying Cho metabolite accurately in breast cancer patients using a clinical 1.5 T scanner.

Từ khóa


Tài liệu tham khảo

Roebuck JR, Cecil KM, Schnall MD, Lenkinski RE (1998) Human breast lesions: characterization with proton MR spectroscopy. Radiology 209:269–275

Kvistad KA, Bakken IJ, Gribbestad IS, Ehrnholm B, Lundgren S, Fjosne HE, Haraldseth O (1999) Characterization of neoplastic and normal human breast tissues with in vivo 1 spectroscopy. J Magn Reson Imaging 10:159–164

Yeung DK, Cheung HS, Tse GM (2001) Human breast lesions: characterization with contrast-enhanced in vivo proton MR spectroscopy-initial results. Radiology 220:40–60

Cecil KM, Schnall MD, Siegelman ES, Lenkinski RE (2001) The evaluation of human breast lesions with magnetic resonance imaging and proton magnetic resonance spectroscopy. Breast Cancer Res Treat 68:45–54

Katz-Brull R, Lavin PT, Lenkinski R (2002) Clinical utility of proton magnetic resonance spectroscopy in characterizing breast lesions. J Natl Cancer Inst 94:1197–1203

Jagannathan NR, Kumar M, Seenu V, Coshic O, Dwivedi SN, Julka PK, Srivastava A, Rath GK (2001) Evaluation of total choline from in-vivo volume localized proton MR spectroscopy and its response to neoadjuvant chemotherapy in locally advanced breast cancer. Br J Cancer 84:1016–1022

Bolan PJ, Meisamy S, Baker EH, Lin J, Emory T, Nelson M, Everson LI, Yee D, Garwood M (2003) In vivo quantification of choline compounds in the breast with 1 Spectroscopy. Magn Reson Med 50:1134–1143

Jacobs MA, Barker PB, Bottomley PA, Bhujwalla Z, Bluemke DA (2004) Proton magnetic resonance spectroscopic imaging of human breast cancer: a preliminary study. J Magn Reson Imaging 19:68–75

Bakken IJ, Gribbestad IS, Singstad TE, Kvistad KA (2001) External standard method for the in vivo quantification of choline-containing compounds in breast tumors by proton MR spectroscopy at 1.5 Tesla. Magn Reson Med 46:189–192

Vanhamme L, van den Boogaart A, Huffel SV (1997) Improved method for accurate and efficient quantification of MRS data with use of prior knowledge. J Magn Reson 129:35–43

Bottomley PA (1987) Spatial localization in NMR spectroscopy in vivo. Ann NY Acad Sci 508:333–348

Ordidge RJ, Bendall MR, Gordon RE, Connelly A (1985). Volume selection for in vivo spectroscopy. In: Govil G, Khetrapal CL, Sarans A (eds). Magnetic resonance in biology and medicine. Tata-McGraw-Hill, New Delhi, pp 387–397

Hasse A, Frahm J, Hanicke W, Mattaei (1985) 1 chemical shift selective (CHESS) imaging. Phys Med Biol 30:431

Doddrell DM, Galloway G, Brooks W, Filed J, Bulsing J, Irving M, Baddeley H (1986) Water signal elimination in vivo, using suppression by mistimed echo and repetitive gradient episodes. J Magn Reson 70:176–180

Naressim A, Couturier C, Devos JM, Janssen M, Mangeat C, de Beer R, Graveron-Demilly D (2001) Java-based graphical user interface for the MRUI quantitation package. MAGMA 12:141–152

Knijn A, de Beer R, van Ormondt D (1992) Frequency-selective quantification in the time domain. J Magn Reson 97:444–450

van den Bos A (1982) Parameter estimation. In: Sydenham PH (eds). Handbook of measurement science, vol 1. Wiley, Chichester 1, pp 33

Longo R, Bampo A, Vidimari R, Magnaldi S, Giorgini A (1995) Absolute quantitation of brain H-1 nuclear magnetic resonance spectra-comparison of different approaches. Invest Radiol 30:199–203

Negengank W (1992) Studies of human tumors by MRS: a review. NMR Biomed 5:303–324

Preul MC, Caramanos Z, Collins DL, Villemure JG, Leblanc R, Olivier A, Pokrupa R, Arnold DL (1996) Accurate, noninvasive diagnosis of human brain tumors by using proton magnetic resonance spectroscopy. Nat Med 2:323–325

Marshall I, Bruce SD, Higinbotham J, MacLullich A, Waardlaw JM, Ferguson KJ, Seckl J (2000) Choice of spectroscopic lineshape model affects metabolite peak areas and area ratios. Magn Reson Med 44:651–657

Stanwell P, Gluch L, Clark D, Tomanek B, Baker L, Giuffre B, Lean C, Malycha P, Mountford C (2005) Specificity of choline metabolites for in vivo diagnosis of breast cancer using 1 at 1.5 T. Eur Radiol 15:1037–1043

Mackinnon WB, Barry PA, Malycha PL, Gillett DJ, Russell P, Lean CL, Doran ST, Barraclough BH, Bilous M, Mountford CE (1997) Fine-needle biopsy specimens of benign breast lesions distinguished from invasive cancer ex vivo with proton MR spectroscopy. Radiology 204:661–666

Cheng LL, Chang W, Smith BL, Gonzalez RG (1998) Evaluating human breast ductal carcinomas with high-resolution magic-angle spinning proton magnetic resonance spectroscopy. J Magn Reson 135:194–202

Gribbestad IS, Sitter B, Lundgren S, Krane J, Axelson D (1999) Metabolite composition in breast tumors examined by proton nuclear magnetic resonance spectroscopy. Anticancer Res 19:1737–1746

Sitter B, Sonnewald U, Spraul M, Fjosne HE, Gribbestad IS (2002) High-resolution magic angle spinning MRS of breast cancer tissue. NMR Biomed 15(5):327–337

Gribbestad IS, Singstad TE, Nilsen G, Fjosne HE, Engan T, Haugen OA, Rinck PA (1993) In vitro proton NMR spectroscopy of extracts from human breast-tumors and noninvolved breast tissue. Anticancer Res 13:1973–1980

Gribbestad IS, Petersen SB, Fjosne HE, Kvinnsland S, Krane J (1994) H-1 NMR spectroscopic characterization of perchloric acid extracts from breast carcinomas and noninvolved breast tissue. NMR Biomed 7:181–194

Ting YT, Sherr D, Degani H (1996) Variations in energy and phospholipid metabolism in normal and cancer human mammary epithelial cells. Anticancer Res 16:1381–1388

Katz-Brull R, Margalit R, Degani H (2001) differential routing of choline in implanted breast cancer and normal organs. Magn Reson Med 46:31–38

Aboagye EO, Bhujwalla ZM (1999) Malignant transformation alters membrane choline phospholipids metabolism of human mammary epithelial cells. Cancer Res 59:80–84

Podo F (1999) Tumour phospholipids metabolism. NMR Biomed 12:976–983

Thông tin
Thông tin xuất bản

Magnetic Resonance Materials in Physics, Biology and Medicine

Thông tin tác giả