Quality of Performance of Assays for Maternal Serum Alphafetoprotein in the United Kingdom: Evidence from the UK External Quality Assessment Scheme 1980–87

Annals of Clinical Biochemistry - Tập 25 Số 3 - Trang 310-318 - 1988
Surajit Gorai1, I Hanning1, R R A Bacon1, W. M. Hunter1
1UK External Quality Assessment Schemes for Peptide Hormones and Related Substances, Immunoassay Section, Department of Clinical Chemistry, University of Edinburgh, 12 Bristo Place, Edinburgh EH1 1ED, UK

Tóm tắt

Between-laboratory agreement in the UK EQAS for maternal serum alphafetoprotein has improved steadily since 1976 and the geometric coefficient of variation is now 8 to 9% at levels of 50 to 150 kU/L. The use of a common standard and commercial assay kits appear to have contributed to this trend. Within-laboratory performance is also generally good, about 50% of participants maintain a bias of less than 5%, together with a scatter of the bias of less than 10%. These data indicate that the quality of assay performance is adequate for the requirements of screening programmes for open neural tube effects. The improvement in laboratory performance is such that between-laboratory agreement is better expressed in kU/L than as multiples of the median. Errors in interpretation of clearly normal or abnormal results appear to be rare (0·4%), and contribute little to overall false positive and negative rates. However. they assume significance as most arc due to avoidable errors.

Từ khóa


Tài liệu tham khảo

Cameron EHD, 1978, Proceedings of the Second Scarborough Conference on Alpha-fetoprotein Serum Screening in Pregnancy, 59

Bacon RRA, 1983, Immunoassays for Clinical Chemistry, 2, 689

10.1136/jcp.34.9.947

10.1016/0092-1157(75)90048-7

10.1093/clinchem/25.5.675

Kirkwood TBL, 1979, Biometrics, 35, 908

Tukey JW, 1977, Exploratory data analysis, USA and Canada

1977, Lancet, 1323

10.1016/S0092-1157(78)80004-3

10.3164/jcbn.2.111

1980, Clin Chim Acta, 105, 9, 10.1016/0009-8981(80)90088-1

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Thông tin xuất bản

Annals of Clinical Biochemistry

Tập 25 Số 3

310-318

Thông tin tác giả