Purinergic receptors are involved in tooth-pulp evoked nocifensive behavior and brainstem neuronal activity

Molecular Pain - Tập 6 - Trang 1-11 - 2010
Kazunori Adachi1,2,3, Kohei Shimizu4, James W Hu5, Ikuko Suzuki6, Hiroshi Sakagami1, Noriaki Koshikawa2,3, Barry J Sessle5, Masamichi Shinoda6,7, Makiko Miyamoto6,8, Kuniya Honda6,9, Koichi Iwata6,7
1Department of Diagnostic & Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Japan
2Department of Pharmacology, Nihon University School of Dentistry, Tokyo, Japan
3Division of Oral and Craniomaxillofacial Research Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan
4Department of Endodontics, Nihon University School of Dentistry, Nihon University School of Dentistry, Tokyo, Japan
5Department of Oral Physiology, Faculty of Dentistry, University of Toronto, Toronto, Canada
6Department of Physiology, Nihon University School of Dentistry, Nihon University School of Dentistry, Tokyo, Japan
7Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Nihon University School of Dentistry, Tokyo, Japan
8Department of Anesthesiology, Nihon University School of Dentistry, Nihon University School of Dentistry, Tokyo, Japan
9Oral and Maxillofacial Surgery Nihon University School of Dentistry, Nihon University School of Dentistry, Tokyo, Japan

Tóm tắt

To evaluate whether P2X receptors are involved in responses to noxious pulp stimulation, the P2X3 and P2X2/3 receptor agonist α,β-methyleneATP (α,β-meATP) was applied to the molar tooth pulp and nocifensive behavior and extracellular-signal regulated kinase (ERK) phosphorylation in trigeminal spinal subnucleus caudalis (Vc), trigeminal spinal subnucleus interpolaris (Vi), upper cervical spinal cord (C1/C2) and paratrigeminal nucleus (Pa5) neurons were analyzed in rats. Genioglossus (GG) muscle activity was evoked by pulpal application of 100 mM α,β-meATP and was significantly larger than GG activity following vehicle (phosphate-buffered saline PBS) application (p < 0.01). The enhanced GG muscle activity following 100 mM α,β-meATP was significantly reduced (p < 0.05) by co-application of 1 mM TNP-ATP (P2X1, P2X3 and, P2X2/3 antagonist). A large number of pERK-LI cells were expressed in the Vc, Vi/Vc, C1/C2 and Pa5 at 5 min following pulpal application of 100 mM α,β-meATP compared to PBS application to the pulp (p < 0.05). The pERK-LI cell expression and GG muscle activity induced by 100 mM α,β-meATP pulpal application were significantly reduced after intrathecal injection of the MAPK/ERK kinase (MEK) inhibitor PD 98059 and by pulpal co-application of 1 mM TNP-ATP (p < 0.05). The present findings suggest that activation of P2X3 and P2X2/3 receptors in the tooth pulp is sufficient to elicit nociceptive behavioral responses and trigeminal brainstem neuronal activity.

Tài liệu tham khảo

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