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Tách tinh, đặc trưng hóa và nghiên cứu kết docking phân tử của peptide ức chế enzym chuyển angiotensin-I (ACE) từ protein thủy phân của cá thu ngắn (Decapterus macrosoma)
Tóm tắt
Tăng huyết áp là một căn bệnh mạn tính đe dọa, trở thành nguyên nhân gây tử vong toàn cầu trong cộng đồng người trưởng thành. Tỷ lệ tử vong ngày càng gia tăng dù đã có nhiều loại thuốc ức chế enzym chuyển angiotensin I (ACE) được đưa ra. Các peptide sinh học có nguồn gốc từ tài nguyên thủy sinh cho thấy hoạt tính ức chế ACE tiềm năng. Mục tiêu của công trình này là báo cáo về quá trình tách tinh và nghiên cứu kết docking phân tử của peptide ức chế ACE được tách từ protein thủy phân phụ phẩm của cá thu ngắn (Decapterus macrosoma) (SWH), được chuẩn bị bằng phương pháp enzym sử dụng alcalase. Quy trình tách tinh bao gồm siêu lọc, lọc gel và sắc ký lỏng hiệu suất cao reverse phase (RP-HPLC). Kết quả cho thấy phần peptide siêu lọc (< 3 kDa) có hoạt tính ức chế ACE cao nhất, tiếp theo là phần 14 qua lọc gel. Phần P thu được từ RP-HPLC, với chuỗi axit amin RGVGPVPAA (IC50 = 0,20 mg/ml) đã được xác định. Về mặt ức chế ACE, biểu đồ Lineweaver–Burk cho thấy peptide SWH thu được có tác dụng như một chất ức chế ACE cạnh tranh. Các nghiên cứu kết docking phân tử cho thấy peptide SWH thể hiện các liên kết hydro và tương tác Pi với ACE với điểm số Z-dock. Những kết quả này cho thấy peptide đã được tinh chế từ thủy phân phụ phẩm của cá thu ngắn có đặc tính hạ huyết áp tiềm năng mà có thể được sử dụng như một thành phần thực phẩm chức năng.
Từ khóa
#Tăng huyết áp #peptide sinh học #phụ phẩm thủy sản #enzym chuyển angiotensin #sắc ký lỏng hiệu suất cao.Tài liệu tham khảo
Abdelhedi O, Nasri R, Jridi M, Mora L, Oseguera-Toledo ME, Aristoy MC, Nasria M (2017) In silico analysis and antihypertensive effect of ACE-inhibitory peptides from smooth-hound viscera protein hydrolysate: Enzyme-peptide interaction study using molecular docking simulation. Process Biochem 58:145–159
Ahn CB, Je JY, Cho YS (2012) Antioxidant and anti-inflammatory peptide fraction from salmon byproduct protein hydrolysates by peptic hydrolysis. Food Res Int 49:92–98
AOAC (2002) Official Methods of Analysis, 16th edn. Association of Official Analytical Chemists, Washington
Barzideh Z, Latiff AA, Gan CY, Abedin MZ, Alias AK (2014) ACE inhibitory and antioxidant activities of collagen hydrolysates from the ribbon jellyfish (Chrysaora sp.). Food Technol Biotech 52:495–504
Chessari S, Thomas R, Polticelli F, Luisi PL (2006) The production of de novo folded proteins by a stepwise chain elongation: a model for prebiotic chemical evolution of macromolecular sequences. Chem Biodivers 3:1202–1210
Cushman DW, Cheung HS (1971) Spectrophotometric assay and properties of the angiotensin- converting enzyme of rabbit lung. Biochem Pharmacol 20:1637–1648
Danijela BK, Barbab FJ, Granatoc D, Galanakis CM, Zoran H, Verica DU, Putnik P (2018) Pressurized hot water extraction (PHWE) for the green recovery of bioactive compounds and steviol glycosides from Stevia rebaudiana Bertoni leaves. Food Chem 254:150–157
Galanakis CM (2020) The Food Systems in the Era of the Coronavirus (COVID-19) Pandemic Crisis. Foods 9:1–10
Ghassem M, Arihara K, Babji AS (2012) Isolation, purification and characterisation of angiotensin I-converting enzyme-inhibitory peptides derived from catfish (Clarias batrachus) muscle protein thermolysin hydrolysates. Int J Food Sci Tech 47:2444–2451
Ghassem M, Babji AS, Said M, Mahmoodani F, Arihara K (2014) Angiotensin I-converting enzyme inhibitory peptides from snakehead fish sarcoplasmic protein hydrolysate. J Food Biochem 38:140–149
Halim NRA, Sarbon NM (2017) A response surface approach on hydrolysis condition of eel (Monopterus Sp.) protein hydrolysate with antioxidant activity. Int Food Res J 24(3):1081–1093
Halim NRA, Yusof HM, Sarbon NM (2016) Functional and Bioactive properties of fish protein hydolysates and peptides: a comprehensive review. Trends Food Sci Tech 51:24–33
Halim NRA, Azlan A, Yusuf HM, Sarbon NM (2018) Antioxidant and anticancer activities of enzymatic eel (Monopterus sp) protein hydrolysate as influenced by different molecular weight. Biocatal Agric Biotechnol 16:10–16
Hooft RW, Sander C, Vriend G (1997) Objectively judging the quality of a protein structure from a Ramachandran plot. Comput Appl Biosci 13(4):425–430
Intarasirisawat R, Benjakul S, Wu J, Visessanguan W (2013) Isolation of antioxidative and ACE inhibitory peptides from protein hydrolysate of skipjack (Katsuwana pelamis) roe. J Funct Foods 5:1854–1862
Ishak NH, Sarbon NM (2017) Optimization of the enzymatic hydrolysis conditions of waste from shortfin (Decapterus macrosoma) for the production of angiotensin I-converting enzyme (ACE) inhibitory peptide using response surface methodology. Int Food Res J 24:1735–1743
Ishak NH, Sarbon NM (2018) A review of protein hydrolysates and bioactive peptides deriving from wastes generated by fish processing. Food Bioprocess Tech 11:2–16
Ketnawa S, Rawdkuen S (2013) Purification and characterization of ace inhibitory peptide from aquatic resources : A review. Int J Pl An Env Sci 3:220–233
Klompong V, Benjakul S, Kantachote D, Shahidi F (2007) Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chem 102(4):1317–1327
Lee JK, Jeon JK, Byun HG (2011) Effect of angiotensin I converting enzyme inhibitory peptide purified from skate skin hydrolysate. Food Chem 125:495–499
Lee JK, Jeon JK, Byun HG (2014) Antihypertensive effect of novel angiotensin I converting enzyme inhibitory peptide from chum salmon (Oncorhynchus keta) skin in spontaneously hypertensive rats. J Funct Foods 7:381–389
Liu X, Zhang M, Zhang C, Liu C (2012) Angiotensin converting enzyme (ACE) inhibitory, antihypertensive and antihyperlipidaemic activities of protein hydrolysates from Rhopilema esculentum. Food Chem 134:34–2140
Ma B, Zhang K, Hendrie C, Liang C, Li M, Doherty-Kirby A, Lajoie G (2003) PEAKS: powerful software for peptide de novo sequencing by tandem mass spectrometry. Rapid Commun Mass Spectrom 17:2337–2342
Najafian L, Babji AS (2015) Isolation, purification and identification of three novel antioxidative peptides from patin (Pangasius sutchi) myofibrillar protein hydrolysates. LWT-Food Sci Technol 60(1):452–461
Nasir SNAM, Sarbon NM (2019) Angiotensin converting enzyme (ACE), antioxidant activity and functional properties of shortfin scad (Decapterus macrosoma) muscle protein hydrolysate at different molecular weight variations. Biocatal Agric Biotechnol 20:101254
Rahmanian N, Jafari SM, Galanakis CM (2014) Recovery and removal of phenolic compounds from olive mill wastewater. J Am Oil Chem Soc 91:1–18
Rasli HI, Sarbon NM (2018) Optimization of enzymatic hydrolysis conditions and characterization of Shortfn scad (Decapterus macrosoma) skin gelatin hydrolysate using response surface methodology. Int Food Res J 25:1541–1549
Rekstena AM, Somasundaramb T, Kjellevolda M, Nordhagena A, Bøkevolla A, Pincusc LM, Rizwand AA, Mamune A, Thilstedc SH, Htutf T, Aakrea I (2020) Nutrient composition of 19 fish species from Sri Lanka and potential contribution to food and nutrition security. J Food Compost 91:103508
Sarbon NM, Badii F, Howell NK (2018) Purification and characterization of antioxidative peptides derived from chicken skin gelatin hydrolysate. Food Hydrocoll 85:311–320
Sarbon NM, Howell NK, Wan Ahmad WAN (2019) Angiotensin-I converting enzyme (ACE) inhibitory peptides from chicken skin gelatin hydrolysate and its antihypertensive effect in spontaneously hypertensive rats. Int Food Res J 26:903–911
Segura-campos MR, Chel-guerrero LA, Betancur-ancona DA (2011) Purification of angiotensin I-converting enzyme inhibitory peptides from a cowpea (Vigna unguiculata) enzymatic hydrolysate. Process Biochem 46:864–872
Shaik MI, Sarbon NM (2020) A review on purification and characterization of anti-proliferative peptides derived from fish protein hydrolysate. Food Rev Int. https://doi.org/10.1080/87559129.2020.1812634
Štich I, Car R, Parrinello M, Baroni S (1989) Conjugate gradient minimization of the energy functional: a new method for electronic structure calculation. Phys Rev B 39:4997
World Health Organization (2013) A global brief on hypertension: silent killer, global public health crisis: World Health Day 2013. World Health Organization.
Wu S, Sun J, Tong Z, Lan X, Zhao Z, Liao D (2012) Optimization of hydrolysis conditions for the production of angiotensin-I-converting enzymeinhibitory peptides and isolation of a novel peptide from Lizard fish (Saurida elongata) muscle protein hydrolysate. Marine Drugs 10:1066–1080
Wu Q, Du J, Jia J, Kuang C (2016) Production of ACE inhibitory peptides from sweet sorghum grain protein using alcalase: Hydrolysis kinetic, purification and molecular docking study. Food Chem 199:140–149