Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidilactici

Wiley - Tập 65 Số 4 - Trang 261-268 - 1988
Arun K. Bhunia1, M.C. Johnson2, Bibek Ray2
1#N# Department of Animal Science, University of Wyoming, Laramie 82071
2Food Microbiology Laboratory, Department of Animal Science, University of Wyoming, Laramie, WY 82071, USA

Tóm tắt

An antimicrobial peptide designated pediocin AcH was isolated from Pediococcus acidilactici strain H. The pediocin AcH was purified by ion exchange chromatography. The molecular weight of pediocin AcH was determined by SDS‐PAGE to be about 2700 daltons. Pediocin AcH was sensitive to proteolytic enzymes, resistant to heat and organic solvents, and active over a wide range of pH. Pediocin AcH exhibited inhibition against several food spoilage bacteria and foodborne pathogens including Staphylococcus aureus, Clostridium perfringens and Listeria monocytogenes. It was bactericidal to sensitive cells and acted very rapidly. The bactericidal effect was not produced by either cell lysis or apparent loss of membrane permeability.

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Wiley

Tập 65 Số 4

261-268

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