Purification and characterization of urease from Ureaplasma urealyticum

Bloomster, 1981, Effect of antibiotics on the dynamics of color change in Ureaplasma urealyticum cultures, J. Clin. Microbiol., 13, 598, 10.1128/JCM.13.3.598-600.1981 Bradford, 1974, A rapid and sensitive method for the quantitation of microgram quantities of protein, utilizing the principle of protein dye binding, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3 Brocklehurst, 1973, Preparation of fully active papain from dried papaya latex, Biochem. J., 133, 573, 10.1042/bj1330573 Creaser, 1985, The purification of urease from Aspergillus nidulans, Int. J. Biochem., 17, 1339, 10.1016/0020-711X(85)90057-6 Delisle, 1977, Multiple forms of urease in cytoplasmic fraction of Ureaplasma urealyticum, J. Bact., 130, 1390, 10.1128/JB.130.3.1390-1392.1977 Eng, 1986, Properties of urease from Ureaplasma urealyticum kinetics, molecular weight and demonstration of multiple enzyme isoelectric point forms, Can. J. Microbiol., 32, 487, 10.1139/m86-089 Eschenbruch, 1982, Experimentally improved reliability of ultrasensitive silver staining of protein in Polyacrylamide gels, Anal. Biochem., 125, 96, 10.1016/0003-2697(82)90387-6 Fishbein, 1969, The structural basis of the catalytic complexity of urease: interacting and interconvertible molecular species (with a note on izoenzyme classes), Ann. N.Y. Acad. Sci., 147, 857, 10.1111/j.1749-6632.1969.tb41296.x Fishbein, 1973, Urease catalysis and structure, J. Biol. Chem., 248, 7870, 10.1016/S0021-9258(19)43270-5 Hausinger, 1986, Purification of a Nickel-containing urease from the rumen anaerobe Selenomonas ruminatium, J. Biol. Chem., 261, 7866, 10.1016/S0021-9258(19)57483-X Kenny, 1983, Inhibition of the growth of Ureaplasma urealyticum by a new urease inhibitor, Flurofamide, Yale J. Biol. Med., 56, 717 Laemmli, 1970, Cleavage of structural proteins during assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 Masover, 1974, Growth of T-strain mycoplasmas in medium without added urea" effects of trace amounts of urease inhibitors, J. Bact., 177, 765, 10.1128/JB.117.2.765-774.1974 Masover, 1975, Localization of enzymes in Ureaplasma urealyticum, J. Bact., 125, 581, 10.1128/JB.125.2.581-587.1976 Montes, 1971, Purification and biochemical characteristics of urease from Proteus rettgeri, Biochim. Biophys. Acta., 242, 230, 10.1016/0005-2744(71)90103-3 Pardee, 1959, The genetic control and cytoplasmatic expression of “inducibility” in the synthesis of B-galactosidase by Escherichia coli, J. Molec. Biol., 1, 165, 10.1016/S0022-2836(59)80045-0 Precious, 1988, Preliminary characterization of the Urease and a 96 kDa surface expressed polypeptide of Ureaplasma urealyticum, J. Gen. Microbiol., 133, 2659 Riddles, 1983, Jack bean urease IV. Determination of thiol and disulfide content. Reversible inactivation of the enzyme by blocking the unique cystein residue, Biochem. Biophys. Acta, 743, 115, 10.1016/0167-4838(83)90424-7 Romano, 1980, Adenosin 5′-triphosphate synthesis induced by urea hydrolysis in Ureaplasma urealyticum, J. Bact., 144, 830, 10.1128/JB.144.2.830-832.1980 Romano, 1979, Urease activity of Ureaplasma urealyticum: some properties of the enzyme, Microbiologica (Bologna), 2, 357 Saada, 1987, Biochemical characteristics of urease for Ureaplasma urealyticum Israel J, Med., 23, 510 Swanberg, 1978, Some characteristics of Ureaplasma urealyticum. Urease activity in simple buffer: effect of metal ions and sulfhydryl inhibitors, J. Gen. Microbiol., 108, 221, 10.1099/00221287-108-2-221 Stemke, 1987, Purification of urease from Ureaplasma urealyticum, Can. J. Microbiol., 33, 857, 10.1139/m87-150 Taylor-Robinson, 1979, Mycoplasmas in human genitourinary infections, Vol. III, 308 Tunon, 1984, Yet another improved silverstaining method for the detection of proteins in polyacrylamide gels, J. Biochem. Biophys. Meth., 9, 171, 10.1016/0165-022X(84)90008-3 Wong, 1973, Single step purification of urease by affinity chromatography, Can. J. Microbiol., 20, 623, 10.1139/m74-095