Protein translocationin vitro: Biochemical characterization of genetically defined translocation components
Tóm tắt
Recent years have seen the convergence of both genetic and biochemical approaches in the study of protein translocation inE. coli. The powerful combination of these approaches is exemplified in the use of anin vitro protein synthesis-protein translocaltion system to analyze the role of genetically defined components of the protein translocation machinery. We describe in this review recent results focusing on the function of thesecA, secB, andsecY gene products and the demonstration of their requirement forin vitro protein translocation. The SecA protein was recently shown to possess ATPase activity and was proposed to be a component of the translocation ATPase. We present a speculative working model whereby the translocator complex is composed of the integral membrane proteins SecY, SecD, SecE, and SecF, forming an aqueous channel in the cytoplasmic membrane, and the tightly associated peripheral membrane protein SecA functioning as the catalytic subunit of the translocator or “protein-ATPase.”
Tài liệu tham khảo
Akiyama, Y., and Ito, K. (1985).EMBO J. 4, 3351–3356.
Akiyama, Y., and Ito, K. (1987).EMBO J. 6, 3465–3470.
Bacallao, R., Crooke, E., Shiba, K., Wickner, W., and Ito, K. (1986).J. Biol. Chem. 261, 12907–12910.
Bankaitis, V. A., and Bassford, P. J., Jr. (1985).J. Bacteriol. 161, 169–178.
Bieker, K. L., and Silhavy, T. J. (1989).Proc. Natl. Acad. Sci. USA 86, 968–972.
Beckwith, J., and Ferro-Novick, S. (1986).Curr. Top. Microbiol. Immunol. 125, 5–27.
Blobel, G., and Dobberstein, B. (1975).J. Cell Biol. 67, 835–851.
Bochkareva, E. S., Lissin, N. M., and Girsohovich, A. S. (1988).Nature (London)336, 254–257.
Brickman, E. R., Oliver, D. B., Garvin, J. L., Kumamoto, C., and Beckwith, J. (1984).Mol. Gen. Genet. 196, 24–27.
Briggs, M., and Gierasch, L. (1986).Adv. Protein Chem. 38, 109–180.
Cabelli, R. J., Chen, L. L., Tai, P. C., and Oliver, D. B. (1988).Cell 55, 683–692.
Cerretti, D. P., Dean, D., Davis, G. R., Bedwell, D. M., and Nomura, M. (1983).Nucleic Acids Res. 11, 2599–2619.
Chen, L. L. and Tai, P. C. (1985).Proc. Natl. Acad. Sci. USA 82, 4384–4388.
Chen, L. L. and Tai, P. C. (1986a).J. Bacteriol. 167, 389–392.
Chen, L. L. and Tai, P. C. (1986b).J. Bacteriol. 168, 828–832.
Chen, L. L. and Tai, P. C. (1987).Nature (London)328, 164–166.
Chen, L. L., Rhoads, D., and Tai, P. C. (1985).J. Bacteriol. 161, 973–980.
Chirico, W. J., Waters, M. G., and Blobel, G. (1988).Nature (London)332, 805–810.
Collier, D. N., Bankaitis, V. A., Weiss, J. B., and Bassford, P. J. (1988).Cell 53, 273–283.
Crooke, E., and Wickner, W. (1987).Proc. Natl. Acad. Sci. USA 84, 5216–5220.
Crooke, E., Brundage, L., Rice, M., and Wickner, W. (1988a).EMBO J. 1, 1831–1835.
Crooke, E., Guthrie, B., Lecker, S., Lill, R., and Wickner, W. (1988b).Cell 54, 1003–1011.
Cunningham, K., and Wickner, W. (1989).Proc. Natl. Acad. Sci. USA 86, 8630–8634.
Cunningham, K., Lill, R., Crooke, E., Rice, M., Moore, K., Wickner, W., and Oliver, D. (1989).EMBO J. 8, 955–959.
Deshaies, R. J., Koch, B. D., Werner-Washburne, M., Craig, E. A., and Scheckman, R. (1988).Nature (London)332, 800–805.
Eilers, M., and Schatz, G. (1988).Cell 52, 481–483.
Ellis, J. (1987).Nature (London)329, 378–379.
Emr, S. D., Hanley-Way, S., and Silhavy, T. J. (1981).Cell 23, 79–88.
Fandl, J., and Tai, P. C. (1987).Proc. Natl. Acad. Sci. USA 84, 7448–7452.
Fandl, J. P., Cabelli, R., Oliver, D., and Tai, P. C. (1988).Proc. Natl. Acad. Sci. USA 85, 8953–8957.
Fikes, J. D., and Bassford, P. J., Jr. (1989).J. Bacteriol. 171, 402–409.
Gannon, P. M., Li, P., and Kumamoto, C. A. (1989).J. Bacteriol. 171, 813–818.
Gardel, C., Benson, S., Hunt, J., Michaelis, S., and Beckwith, J. (1987).J. Bacterol. 169, 1286–1290.
Geller, B. L., and Green, H. M. (1989).J. Biol. Chem. 264, 16465–16469.
Geller, B. L., Moova, N. R., and Wickner, W. (1986).Proc. Natl. Acad. Sci. USA 83, 4219–4222.
Hemmingsen, S. M., Woolford, C., van der Vies, S. M., Tilly, K., Dennis, D. T., Georgopoulos, C. P., Hendrix, R., and Ellis, R. J. (1988).Nature (London)333, 330–334.
Hendrix, R. W. (1979).J. Mol. Biol. 129, 375–392.
Hsu, C. M., and Rosen, B. P. (1989).J. Biol. Chem. 264, 17349–17354.
Kawasaki, H., Matsuyama, S., Sasaki, S., Akita, S., and Mizushima, S. (1989).FEBS Lett. 242, 431–434.
Kinoshita, N., Unemoto, T., and Kobayushi, H. (1984).J. Bacteriol. 160, 1074–1077.
Kumamoto, C. A. (1989).Proc. Natl. Acad. Sci. USA 86, 5320–5324.
Kumamoto, C. A., and Beckwith, J. (1983).J. Bacteriol. 154, 253–260.
Kumamoto, C. A., and Gannon, P. M. (1988).J. Biol. Chem. 263, 11554–11558.
Kumamoto, C. A., Chen, L. L., Fandl, J. P., and Tai, P. C. (1989).J. Biol. Chem. 264, 2242–2249.
Lecker, S., Lill, R., Ziegelhoffer, T., Georgopoulos, C., Bassford, P. J., Jr., Kumamoto, C. A., and Wickner, W. (1989).EMBO J. 8, 2703–2709.
Lee, C. A., and Beckwith, J. (1986).J. Bacteriol. 166, 878–883.
Lee, C. A., Fournier, M. J., and Beckwith, J. (1985).J. Bacteriol. 161, 1156–1161.
Lill, R., Cunningham, K., Brundage, L., Ito, K., Oliver, D., and Wickner, W. (1989).EMBO J. 8, 961–966.
Lin, J. J-C., Kanazawa, H., Ozals, J., and Wu, H. C. (1978).Proc. Natl. Acad. Sci. USA 75, 4891–4895.
Meyer, D. I., Krause, E., and Dobberstein, B. (1982).Nature (London)297, 647.
Muller, M., and Blobel, G. (1984a).Proc. Natl. Acad. Sci. USA 81, 7421–7425.
Muller, M., and Blobel, G. (1984b).Proc. Natl. Acad. Sci. USA 81, 7737–7741.
Ohno-Iwashita, Y., and Wickner, W. (1983).J. Biol. Chem. 258, 1895–1900.
Oliver, D. (1985).Annu. Rev. Microbiol. 39, 615–648.
Oliver, D. B., and Beckwith, J. (1981).Cell 25, 765–772.
Oliver, D. B., and Beckwith, J. (1982).Cell 30, 311–319.
Oliver, D. B., and Liss, L. (1985).J. Bacteriol. 161, 817–819.
Randall, L., and Hardy, S. J. S. (1986).Cell 46, 921–928.
Randall, L., Hardy, S. J. S., and Thom, J. (1987).Ann. Rev. Microbiol. 41, 507–541.
Ray, P., Dev, I., MacGregor, C., and Bassford, P. (1986).Curr. Topic Microbiol. Immunol. 125, 75–102.
Rhoads, D., Tai, P. C., and Davis, B. D. (1984).J. Bacteriol. 159, 63–70.
Riggs, P. D., Derman, A. I., and Beckwith, J. (1988).Genetics 118, 571–579.
Rollo, E. E., and Oliver, D. B. (1988).J. Bacteriol. 170, 3281–3282.
Schatz, P. J., Riggs, P. D., Jacq, A., Fath, M. J., and Beckwith, J. (1989).Genes Dev. 3, 1035–1044.
Schmidt, M. G., and Oliver, D. B. (1989).J. Bacteriol. 171, 643–649.
Schmidt, M., Rollo, E., Grodberg, J., and Oliver, D. (1988).J. Bacteriol. 170, 3404–3414.
Shiba, K., Ito, K., Yura, T., and Cerretti, D. (1984).EMBO J. 3, 631–635.
Shiba, K., Ito, K., and Yura, T. (1986).J. Bacteriol. 166, 849–856.
Silhavy, T. J., Benson, S. A., and Emr, S. D. (1983).Microbiol. Rev. 47, 313–344.
Singer, S. J., Maher, P. M., and Yaffe, M. P. (1987).Proc. Natl. Acad. Sci. USA 84, 1015–1019.
Stader, J., Gansheroff, D., and Silhavy, T. J. (1989).Genes Dev. 3, 1045–1052.
Sternweis, P. C., and Smith, J. B. (1980).Biochemistry 19, 526–531.
Subramanian, A. R., Haase, C., and Giesen, M. (1976).Eur. J. Biochem. 671, 591–601.
Tai, P. C. (1986).Curr. Top. Microbiol. Immunol. 125, 43–58.
Tai, P. C. (1990). InBacterial Energetics (Krulwich, T., ed.), Academic Press, Orlando, Florida. In press.
Tian, G., Wu, H. C., Ray, P. H., and Tai, P. C. (1989).J. Bacteriol. 171, 1987–1997.
Verner, K., and Schatz, G. (1988).Science 241, 1307–1313.
Walter, P., and Blobel, G. (1981).J. Cell Biol. 91, 557–561.
Watanabe, M., and Blobel, G. (1989a).Proc. Natl. Acad. Sci. USA 86, 2248–2252.
Watanabe, M., and Blobel, G. (1989b).Proc. Natl. Acad. Sci. USA 86, 1895–1899.
Watanabe, M., and Blobel, G. (1989c).Proc. Natl. Acad. Sci. USA 86, 2728–2732.
Watanabe, M., and Blobel, G. (1989d).Cell 58, 695–705.
Watanabe, T., Hayashi, S., and Wu, H. C. (1988).J. Bacteriol. 170, 4001–4007.
Weiss, J. B., Ray, P. H., and Bassford, P. J. (1988).Proc. Natl. Acad. Sci. USA 85, 8978–8982.
Weng, Q. P., Chen, L. L., and Tai, P. C. (1988).J. Bacteriol. 170, 126–131.
Wu, H., and Tai, P. (1986) (editors).Protein Secretion and Export in Bacteria (Curr. Top. Microb. Immunol.), Vol. 125, Springer-Verlag.
Yamada, H., Matsuyama, S., Tokuda, H., and Mizushima, S. (1989a).J. Biol. Chem. 264, 18577–18581.
Yamada, H., Tokuda, H., and Mizushima, S. (1989b).J. Biol. Chem. 264, 1723–1728.
Yamane, K., Ichihara, S., and Mizushima, S. (1987).J. Biol. Chem. 262, 2358–2362.
Yamane, K., Matsuyama, S. I., and Mizushima, S. I. (1988).J. Biol. Chem. 263, 5368–5372.