Protein dynamics from single-molecule fluorescence intensity correlation functions

Journal of Chemical Physics - Tập 131 Số 9 - 2009
Irina V. Gopich1, Daniel Nettels2, Benjamin Schuler2, Attila Szabó1
1National Institutes of Health 1 Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, , Bethesda, Maryland 20892, USA
2Biochemisches Institut, Universität Zürich 2 , Winterthurerstr 190, 8057 Zürich, Switzerland

Tóm tắt

Fluorescence intensity correlation functions contain information about photophysical and conformational dynamics. We propose and implement a simple procedure to analyze such functions measured in the presence of resonance energy transfer. When there is a separation of time scales and the conformational dynamics is modeled as diffusion in the potential of mean force along the interdye distance, we obtain an analytic expression for the conformational correlation time. This can be used to find the diffusion coefficient describing conformational fluctuations given the photon count rate and equilibrium distribution.

Từ khóa


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