Protein Detection Methods in Proteomics Research

Bioscience Reports - Tập 25 Số 1-2 - Trang 19-32 - 2005
Reiner Westermeier1, Rita Marouga2
1GE Healthcare Bio-Sciences, Munzinger Strasse 9, D-79111, Freiburg, Germany
2GE Healthcare Bio-Sciences, Björkgatan 30 S-751 82, Uppsala, Sweden

Tóm tắt

In proteomics research chemical as well as physical methods are used to detect proteins subsequently to their separation. Physical methods are mostly applied after chromatography. They are either based on spectroscopy like light absorption at certain wavelengths or mass determination of peptides and their fragments with mass spectrometry. Chemical methods are used after two-dimensional electrophoresis and employ staining with organic dyes, metal chelates, fluorescent dyes, complexing with silver, or pre-labeling with fluorophores. In some cases autoradiography is still used. Since all of these techniques are very different in terms of sensitivity, their usefulness for quantitative determinations varies significantly. This review will describe the various protein detection methods applied to electrophoresis gels.

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Bioscience Reports

Tập 25 Số 1-2

19-32

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