Protective Role of Mentha arvensis Aqueous Extract against Manganese Induced Toxicity by Reducing Mn Translocation and Promoting Antioxidative Defense in growing Indica Rice Seedlings
Tóm tắt
Mentha arvensis L. (Ma) leaf extract, a wide source of natural antioxidants is widely used to protect animal cells against oxidative stress. In the current study, we have studied the ameliorative effects of Ma leaf extract on Mn induced oxidative stress in rice (Oryza sativa L.) seedlings. Hydroponically grown rice seedlings treated with 1.5 mM MnCl2 showed a decline in vigour and contents of photosynthetic pigments, increased production of reactive oxygen species (O2˙‾, H2O2, and.OH), lipid peroxidation, protein thiol and carbonyl contents along with increased activity of antioxidative enzymes- superoxide dismutase (SOD), guaiacol peroxidase (GPX) and ascorbate peroxidase (APX). The activity of catalase (CAT), however, declined in the seedlings after prolonged Mn treatment of 8 days. Interestingly exogenous application of Ma leaf extract (638.4 mg ml-1) effectively restored the Mn-induced decline in seedling vigour, photosynthetic pigments, increased levels of H2O2, lipid peroxides and altered activities of antioxidative enzymes. The results indicate that exogenous application of Ma leaf extract significantly lowers the toxic effects of Mn in rice seedlings by modulating Mn translocation and reducing oxidative stress.
Tài liệu tham khảo
Allen SE, Grimshaw HM, Rowland AP (1986) Chemical analysis. In: Mooren PD, Chapman SB (Eds.) Methods in Plant Ecology, Blackwell Scientific Publication, Oxford: 285–344
Arumugam P, Ramamurthy P, Santhiya ST, Ramesh A (2006) Antioxidant activity measured in different solvent fractions obtained from Mentha spicata L.: an analysis by ABTS+ decolorization assay. Asia Paci J Clin Nutri 15(1): 119–224
Baliga MS, Jagetia GC, Rao SK, Babu S (2003) Evaluation of nitric oxide scavenging activity of certain spices in vitro: a preliminary study. Food/Nahrung 47(4): 261–264
Baliga MS, Rao S (2010) Radioprotective potential of mint: a brief review. Journal of Cancer Research and Therapeutics 6 (3): 255–262
Barceloux DG (1999) Manganese. Clinical Toxicology 37: 293–307
Bartoli CG, Gomez F, Martinez DE, Guiamet JJ (2004) Mitochondria are the main target for oxidative damage in leaves of wheat (Triticum aestivum L.). J Exp Bot 55: 1663–1669
Beauchamp CO, Fridovich I (1971) Superoxide dismutase: improved assay and an assay applicable to acrylamide gels. Anal Biochem 44: 176–287
Beers RF, Sizer IW (1952) Colorimetric method for estimation of catalase. J Biol Chem 195: 133–139
Bhattacharjee SK (2000) Handbook of Medicinal Plants. Aavishkar Publishers
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72(1): 248–254
Buchanan B, Grusen W, Jones R (2000) Biochemistry and Molecular biology of Plants. American Society of Plant Physiologists Maryland, p.1367
Coutinho HD, Costa JG, Lima EO, Falcão–Silva VS, Siqueira–Júnior JP (2008) Enhancement of the antibiotic activity against a multiresistant Escherichia coli by Mentha arvensis L. and chlorpromazine. Chemotherapy 54(4): 328–330
de Varennes A, Carneiro JP, Goss MJ (2011) Characterization of manganese toxicity in two species of annual medics. J Plant Nut 24: 1947–1955
Demirevska–Kepova K, Simova–Stoilova L, Stoyanova Z, Hölzer R, Feller U (2004) Biochemical changes in barley plants after excessive supply of copper and manganese. Environ Exp Bot 52(3): 253–266
Dorman HD, Kosar M, Kahlos K, Holm Y, Hiltunen R (2003) Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. J Agri Food Chem 51(16): 4563–4569
Egley GH, Paul RN, Vaughn KC, Duke SO (1983) Role of peroxidase in the development of water impermeable seed coats in Sida spinosa L. Planta 157: 224–232
El–Jaoual T, Cox DA (1998) Manganese toxicity in plants. J of Plant Nut 21(2): 353–386
Fecht–Christoffers MM, Braun HP, Lemaitre–Guillier C, VanDorsselaer A, Horst WJ (2003) Effect of manganese toxicity on the proteome of the leaf apoplast in cowpea. Plant Physiol 133(4): 1935–1946
Frahry G, Schopfer P (2001) NADH stimulated, cyanide resistant superoxide production in maize coleoptiles analyzed with tetrazolium based assay. Planta 212: 175–183
Gonzales A, Lynch J (1999) Subcellular tissue Mn compartmentation in bean leaves under Mn toxicity stress. Aust J Plant Physiol 26: 811–822
González A, Steffen KL, Lynch JP (1998) Light and excess manganese implications for oxidative stress in common bean. Plant Physiol 118(2): 493–504
Halliwell B, John Gutteridge MC (1981) Formation of a thiobarbituric acid reactive substance from deoxyribose in the presence of Iron salts. FEBS letters 128(2): 347–352
Horiguchi T (1988) Mechanism of manganese toxictty and tolerance of plants VII. Effect of light intensity on manganese–induced chlorosis. J Plant Nutri 11(3): 235–246
Horst WJ (1988) The Physiology of Manganese Toxicity. In Manganese in Soils and Plants, Springer Netherlands, pp. 175–188
Jackson LJ, Kalff J, Rasnnussen JB (1993) Sediment pH and redox potential affect the bioavailability of Al, Cu, Fe, Mn, and Zn to rooted aquatic macrophytes. Cand J of Fish and Aquat Sci 50(1): 143–148
Jana S, Choudhuri MA (1981) Glycolate metabolism of three submerged aquatic angiosperms during aging. Aquat Bot 12: 345–354
Kenten RH, Mann PJ (1950) The oxidation of manganese by peroxidase systems. Biochem Journal 46(1): 67–73
Kochian LV, Hoekenga OA, Magalhaes JV, Pineros MA (2009) Maize Aluminum Tolerance. In: Wessler S, Bennetzen J (eds.) Handbook of Maize: its Biology. Springer–Verlag, Berlin Heidelberg, pp. 367–380
Kosar M, Dorman HD, Can Baser KH, Hiltunen R (2004) Screening of free radical scavenging compounds in water extracts of Mentha samples using a postcolumn derivatization method. J Agri Food Chem 52(16): 5004–5010
Kováčik J, Babula P, Hedbavny J, Švec P (2014) Manganeseinduced oxidative stress in two ontogenetic stages of chamomile and amelioration by nitric oxide. Plant Science 215: 1–10
Kok LJ, & Kuiper PJ (1986) Effect of short–term dark incubation with sulfate, chloride and selenate on the glutathione content of spinach leaf discs. Physiol Plant 68(3): 477–482
Kowti R, Vishwanath S, Shivakumar SI, Vedamurthy J, Abdul NK (2013) Hepatoprotective and antioxidant activity of ethanol extract of Mentha arvensis L. leaves against carbon tetrachloride induced hepatic damage in rats. Inter J Pharm Tech Res 5(2): 426–430
Labra M, Gianazza E, Waitt R, Eberini I, Sozzi A, Regondi S, Grassi F, Agradi E (2006) Zea mays L. protein changes in response to potassium dichromate treatments. Chemosphere 62(8): 1234–1244
Lange BM, Croteau R (1999) Genetic engineering of essential oil production in mint. Current Opinion in Plant Biology 2(2): 139–144
Lavres Junior J, Reis AR, Rossi ML, Cabral CP, Nogueira ND, Malavolta E (2010) Changes in the ultrastructure of soybean cultivars in response to manganese supply in solution culture. Scientia Agricola 67(3): 287–294
Levine A, Tenhaken R, Dixon R, & Lamb C (1994) H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79(4): 583–593
Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148: 350–382
Londonkar RL, Poddar PV (2009) Studies on activity of various extracts of Mentha arvensis L. against drug induced gastric ulcer in mammals. World Journal of Gastrointestinal Oncology 1(1): 82–88
Maksimović JD, Mojović M, Maksimović V, Römheld V, Nikolic M (2012) Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast. J Exp Bot 63(7): 2411–2420
Marschner H (1991) Mechanisms of adaptation of plants to acid soils. In Plant–Soil Interactions at low pH. Springer Netherlands 683–702
Marschner M (1995) Mineral Nutrition of Higher Plants, 2nd Edn. Academic Press, London
Millaleo R, Reyes–Díaz M, Ivanov AG, Mora ML, Alberdi M (2010) Manganese as essential and toxic element for plants: transport, accumulation and resistance mechanisms. J Soil Sci Plant Nutr 10 (4): 470–481
Mishra HP, Fridovich I (1972) The role of superoxide anion in auto–oxidation of the epinephrine and sample assay for SOD. J Biol Chem 247: 3170–3175
Møller IM, Jensen PE, Hansson A (2007) Oxidative modifications to cellular components in plants. Annu. Rev. Plant Biol. 58: 459–481
Mukhopadhyay M, Sharma A (1991) Manganese in cell metabolism of higher plants. Bot Rev 57: 117–149
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate–specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22(5): 867–880
Pandey P, Srivastava RK, Dubey RS (2014) Water deficit and aluminum tolerance are associated with a high antioxidative enzyme capacity in Indica rice seedlings. Protoplasma 251(1): 147–160
Paul A, Hauck M, Fritz E (2003) Effects of manganese on element distribution and structure in thalli of the epiphytic lichens Hypogymniaphysodes and Lecanoraconizaeoides. Environ Exp Bot 50: 113–124
Pompella A, Maellaro E, Casini AF, Comporti M (1987) Histochemical detection of lipid peroxidation in the liver of bromobenzene–poisoned mice. Am J Pathol 129: 295–301
Procházková D, Boušová I, Wilhelmová N (2011) Antioxidant and prooxidant properties of flavonoids. Fitoterapia 82(4): 513–523
Rajpoot R, Rani A, Srivastava RK, Pandey P, Dubey RS (2015) Terminalia arjuna bark extract alleviates nickel toxicity by suppressing its uptake and modulating antioxidative defence in rice seedlings. Protoplasma 253(6): 1449–1462
Scalbert A, Johnson IT, Saltmarsh M (2005) Polyphenols: antioxidants and beyond. The Am J Clin Nutr 81(1): 215S–217S
Schutzendubel A, Schwanz P, Teichmann T, Gross K, Langenfeld–Heyser R, Godbold DL, Polle A (2001) Cadmium–induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in Scots pine roots. Plant Physiol 127: 887–898
Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, Vol. 2012, Article ID 217037, 26 pages, doi:10.1155/2012/217037
Sheng H, Zeng J, Yan F, Wang X, Wang Y, Kang H, Fan X, Sha L, Zhang H, Zhou Y (2015) Effect of exogenous salicylic acid on manganese toxicity, mineral nutrients translocation and antioxidative system in polish wheat (Triticum polonicum L.). Acta Physiol Plant 37(2): 1–11
Shenker M, Plessner OE, Tel–Or E (2004) Manganese nutrition effects on tomato growth, chlorophyll concentration, and superoxide dismutase activity. J Plant Physiol 161: 197–202
Shi Q, Zhu Z (2008) Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber. Environ Exp Bot 63(1): 317–326
Shi QH, Bao ZY, Zhu ZJ, He Y, Qian QQ, Yu JQ (2005a) Silicon–mediated alleviation of Mn toxicity in Cucumis sativus in relation to activities of superoxide dismutase and ascorbate peroxidase. Phytochemistry 66: 1551–1559
Shi QH, Zhu ZJ, Xu M, Qian QQ, Yu JQ (2005b) Effect of excess manganese on the antioxidant system in Cucumis sativus L. under two light intensities. Environ Exp Bot 58: 197–205
Singh VP (2005). Metal toxicity and tolerance in plants and animals. Sarup and Sons, New Delhi, India
Sparrow LA, Uren NC (2014) Manganese oxidation and reduction in soils: effects of temperature, water potential, pH and their interactions. Soil Res 52(5): 483–494
Srivastava RK, Pandey P, Rajpoot R, Rani A, Gautam A, Dubey RS (2015) Exogenous application of calcium and silica alleviates cadmium toxicity by suppressing oxidative damage in rice seedlings. Protoplasma. 252(4): 959–975
Srivastava S, Dubey RS (2011) Manganese–excess induces oxidative stress, lowers the pool of antioxidants and elevates activities of key antioxidative enzymes in rice seedlings. Plant Growth Regulation 64(1): 1–6
Stiborová M, Ditrichová M, BŘEzinová A (1987) Effect of heavy metal ions on growth and biochemical characteristics of photosynthesis of barley and maize seedlings. Biologia Plantarum 29(6): 453–467
Takahama U (1993) Redox state of ascorbic acid in the apoplast of stems of Kalanchoë daigremontiana. Physiologia Plantarum 89(4): 791–798
Takahashi MA, Asada K (1983) Superoxide anion permeability of phospholipid membranes and chloroplast thylakoids. Arch Biochem Biophys 226: 558–566
Thordal–Christensen H, Zhang Z, Wei YD, Collinge DB (1997) Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barleypowdery mildew interaction. Plant J 11: 1187–1194
Triantaphyllou K, Blekas G, Boskou D (2001) Antioxidative properties of water extracts obtained from herbs of the spices Lamiaceae. Int. J Food Sci and Nutri 52: 313–317
Verma S, Dubey RS (2003) Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Science 164(4): 645–655
Verma SM, Arora H, Dubey R (2003) Anti–inflammatory and sedative–hypnotic activity of the methanolic extract of the leaves of Mentha arvensis. Anc Sci Life 23(2): 95–99
Wang YX, Wu P, Wu YR, Yan XL (2002) Molecular marker analysis of manganese toxicity tolerance in rice under greenhouse conditions. Plant and Soil 238(2): 227–233
Yoshida S, Forno DA, Cock JH, Gomez KA (1976) Laboratory manual for physiological studies of rice, 3rd edn. International Rice Research Institute, Philippines, p. 83