Protection against Lethal Leptospirosis after Vaccination with LipL32 Coupled or Coadministered with the B Subunit of Escherichia coli Heat-Labile Enterotoxin

American Society for Microbiology - Tập 19 Số 5 - Trang 740-745 - 2012
André Alex Grassmann1, Samuel Rodrigues Félix2, Carolina Ximendes dos Santos2, Marta Gonçalves Amaral2, Amilton Clair Pinto Seixas Neto2, Michel Q. Fagundes2, Fabiana K. Seixas2, Éverton F. da Silva3, Fabrício Rochedo Conceição2, Odir Antônio Dellagostin2
1Unidade de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Brazil
2aUnidade de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Brazil
3bFaculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, Brazil

Tóm tắt

ABSTRACT

Leptospirosis, a worldwide zoonosis, lacks an effective, safe, and cross-protective vaccine. LipL32, the most abundant, immunogenic, and conserved surface lipoprotein present in all pathogenic species ofLeptospira, is a promising antigen candidate for a recombinant vaccine. However, several studies have reported a lack of protection when this protein is used as a subunit vaccine. In an attempt to enhance the immune response, we used LipL32 coupled to or coadministered with the B subunit of theEscherichia coliheat-labile enterotoxin (LTB) in a hamster model of leptospirosis. After homologous challenge with 5× the 50% lethal dose (LD50) ofLeptospira interrogans, animals vaccinated with LipL32 coadministered with LTB and LTB::LipL32 had significantly higher survival rates (P< 0.05) than animals from the control group. This is the first report of a protective immune response afforded by a subunit vaccine using LipL32 and represents an important contribution toward the development of improved leptospirosis vaccines.

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