Profiling maternal plasma microRNA expression in early pregnancy to predict gestational diabetes mellitus

International Journal of Gynecology & Obstetrics - Tập 130 - Trang 49-53 - 2015
Yanan Zhu1, Fei Tian1, Hailing Li2, Youxia Zhou1, Jiafeng Lu3, Qinyu Ge1
1Key Lab for Child Development and Learning Science, Ministry of Education, Research Center for Learning Science, Southeast University, Nanjing, China
2Department of Gynecology and Obstetrics, Zhongda Hospital, Southeast University, Nanjing, China
3State Key Lab of Bioelectronics, Southeast University, Nanjing, China

Tóm tắt

AbstractObjectiveTo profile the differential expression of plasma miRNAs in gestational diabetes mellitus (GDM).MethodsIn a pilot study conducted at a tertiary hospital in China between 2010 and 2014, peripheral blood samples were collected from women at 16–19 weeks of pregnancy. Pooled samples from 10 women who were subsequently diagnosed with GDM and from 10 healthy controls were used to construct two small RNA libraries. High‐throughput sequencing was performed, and differentially expressed miRNAs were validated by quantitative real‐time polymerase chain reaction (qRT‐PCR), followed by target prediction, Gene Ontology analysis, and pathway identification.ResultsSequencing revealed 32 miRNAs that were differentially expressed in GDM, including 12 miRNAs that were upregulated and 20 that were downregulated. Differential expression of five upregulated miRNAs (hsa‐miR‐16‐5p, hsa‐miR‐17‐5p, hsa‐miR‐19a‐3p, hsa‐miR‐19b‐3p, hsa‐miR‐20a‐5p) was confirmed by qRT‐PCR. Target prediction showed that the major targets of these miRNAs were associated with insulin resistance and abnormal pregnancies.ConclusionThe five miRNAs that were differentially expressed in GDM could serve as noninvasive biomarkers. The results also provide insights into the molecular mechanisms that underlie GDM, thereby contributing to the diagnosis and treatment of this disease.

Tài liệu tham khảo

10.1016/j.diabres.2010.04.001 10.1056/NEJMoa0707943 10.1016/j.ajog.2008.06.031 Tieu J., 2010, Screening and subsequent management for gestational diabetes for improving maternal and infant health, Cochrane Database Syst Rev, 7, CD007222 10.1111/j.1464-5491.2011.03236.x 10.1152/physrev.00006.2010 Coordes A., 2014, Role of miRNA in malignoma of the head and neck, Laryngorhinootologie, 93, 201 10.1016/S0092-8674(04)00045-5 10.1038/nature02871 10.1016/j.clinbiochem.2013.01.026 10.1161/CIRCRESAHA.110.227496 10.2337/dc11-S011 10.1101/gr.7.10.986 10.1002/ijc.28757 10.2337/dc07-s225 10.1111/j.1742-4658.2011.08261.x 10.1038/onc.2012.327 10.1038/nrm1837 10.1074/jbc.M113.544999 10.1007/s00125-006-0552-5 10.1016/j.lfs.2005.11.021 10.1111/j.1523-1755.2004.00476.x 10.1016/j.placenta.2011.09.011 10.1371/journal.pone.0097632 10.2337/db09-1061 10.3390/ijms14035519 10.1210/jc.2011-3131 10.1177/1933719109336614
Thông tin
Thông tin xuất bản

International Journal of Gynecology & Obstetrics

Tập 130

49-53

Thông tin tác giả