Production, partial purification and characterization of a proteoglycan bioemulsifier from an oleaginous yeast
Tóm tắt
In this study, Meyerozyma caribbica, an indigenously isolated oleaginous yeast, produced in media containing glucose a bioemulsifier that was partially characterized as a proteoglycan based on preliminary analysis. Optimization of carbon:nitrogen (C:N) ratio revealed 30:1 as the suitable ratio for enhanced production. Apart from higher emulsification activity (E24: 70–80%), this molecule showed strong emulsion stability over a wide range of pH (2.0–9.0), salinity (0.05%—10%, w/v) and temperature (− 80 °C to + 50 °C). The current study emphasizes on the determination of critical media parameters for improved and stable bioemulsifier production coupled with partial characterization and identification of the molecule. Thus, a proteoglycan-based bioemulsifier with such a stable emulsifying property can serve as a versatile and potential component in food, cosmetics and pharmaceutical formulations.
Tài liệu tham khảo
Van Hamme JD, Singh A, Ward OP (2006) Physiological aspects. Part 1 in a series of papers devoted to surfactants in microbiology and biotechnology. Biotechnol Adv 24(6):604–620. https://doi.org/10.1016/j.biotechadv.2006.08.001
Sen R (2010) Biosurfactants: advances in experimental medicine and biology, vol 672. Springer-Verlag, New York
Satpute SK, Banat IM, Dhakephalkar PK, Banpurkar AG, Chopade BA (2010) Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms. Biotechnol Adv 28(4):436–450. https://doi.org/10.1016/j.biotechadv.2010.02.006
Chopra J, Dineshkumar R, Bhaumik M, Dhanarajan G, Kumar R, Sen R (2016) Integrated in situ transesterification for improved biodiesel production from oleaginous yeast: a value proposition for possible industrial implication. RSC Adv 6(74):70364–70373. https://doi.org/10.1039/C6RA14003C
Kumar R, Dhanarajan G, Bhaumik M, Chopra J, Sen R (2017) Performance evaluation of a yeast biorefinery as a sustainable model for co-production of biomass, bioemulsifier, lipid, biodiesel and animal-feed components using inexpensive raw materials. Sustain Energ Fuels 1(4):923–931. https://doi.org/10.1039/C7SE00010C
Johnson V, Singh M, Saini VS, Adhikari DK, Sista V, Yadav NK (1992) Bioemulsifier production by an oleaginous yeast Rhodotorula glutinis IIP 30. Biotechnol Lett 14(6):487–490. https://doi.org/10.1007/BF01023172
Dubois M, Gilles K, Hamilton JK, Rebers PA, Smith F (1951) Colorimetric method for determination of sugars. Nature 168(4265). https://www.ncbi.nlm.nih.gov/pubmed/?term=SMITH%20F%5BAuthor%5D&cauthor=true&cauthor_uid=14875032. Accessed 4 Sept 2019
Lowry OH, Rosebrough NJ, Al F, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193(1):265–275
Satpute SK, Banpurkar AG, Dhakephalkar PK, Banat IM, Chopade BA (2010) Methods for investigating biosurfactants and bioemulsifiers: a review. Crit Rev Biotechnol 30(2):127–144. https://doi.org/10.3109/07388550903427280
Vilim V, Krajickova J (1991) Electrophoretic separation of large proteoglycans in large pore polyacrylamide gradient gels (1.32%-10% T) and a one step procedure for simultaneous staining of proteins and proteoglycans. Anal Biochem 197:34–39
Castellane TCL, Campanharo JC, Coutinho LACID, Lopes ÉM, Lemos MVF, Lemos EGM (2017) Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate. Int J Biol Macromol 96:361–369. https://doi.org/10.1016/j.ijbiomac.2016.11.123
Violetta MR, Mazzoli R, Barello C, Fattori P, Giuffrida MG, Pessione E (2014) Combining LC-MS/MS, PMF and N-terminal amino acid sequencing for multiplexed characterization of a bacterial surfactant glycoprotein biosynthesized by Acinetobacter radioresistens S13. RSC Adv 4(21):10918–10927. https://doi.org/10.1039/C4RA00692E
Cooper DG, Goldenberg BG (1987) Surface-active agents from two Bacillus species. Appl Environ Microbiol 53(2):224–229
Uzoigwe C, Burgess JG, Ennis CJ, Rahman PKSM (2015) Bioemulsifiers are not biosurfactants and require different screening approaches. Front Microbiol 6:245. https://doi.org/10.3389/fmicb.2015.00245
Fan Y, Tao W, Huang H, Li S (2017) Characterization of a novel bioemulsifier from Pseudomonas stutzeri. World J Microbiol Biotechnol 33:161. https://doi.org/10.1007/s11274-017-2326-2
Lukondeh T, Ashbolt NJ, Rogers PL (2003) Evaluation of Kluyveromyces marxianus FII 510700 grown on a lactose-based medium as a source of a natural bioemulsifier. J Ind Microbiol Biotechnol 30(12):715–720. https://doi.org/10.1007/s10295-003-0105-6
Amaral PFF, da Silva JM, Lehocky M, Barros-Timmons AMV, Coelho MAZ, Marrucho IM, Coutinho JAP (2006) Production and characterization of a bioemulsifier from Yarrowia lipolytica. Process Biochem 41(8):1894–1898. https://doi.org/10.1016/j.procbio.2006.03.029
Cirigliano MC, Carman GM (1985) Purification and characterization of Liposan, a bioemulsifier from Candida lipolytica. Appl Environ Microbiol 50(4):846–850
Sarubbo LA, Galba JMDL, Campos-Takaki MD (2006) Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabarta UCP 1002. Electron J Biotechnol. https://doi.org/10.2225/vol9-issue4-fulltext-6
Lorliam W, Akaracharanya A, Suzuki M, Ohkuma M, Tanasupawat S (2013) Diversity and fermentation products of xylose-utilizing yeasts isolated from buffalo feces in Thailand. Microbes Environ 28(3):354–360. https://doi.org/10.1264/jsme2.me13023
Costa E, Teixidó N, Usall J, Atarés E, Viñas I (2002) The effect of nitrogen and carbon sources on growth of the biocontrol agent Pantoea agglomerans strain CPA-2. Lett Appl Microbiol 35:117–120
Cooper DG, Paddock DA (1984) Production of a biosurfactant from Torulopsis bombicola. Appl Environ Microbiol 47(1):173–176
Sarubbo LA, Marçal MC, Neves ML, Silva MP, Porto AL, Campos-Takaki GM (1997) Bioemulsifier production in batch culture using glucose as carbon source by Candida lipolytica. Appl Biochem Biotechnol 95(1):59–67
Gargouri B, Contreras MD, Ammar S, Segura-Carretero A, Bouaziz M (2017) Biosurfactant production by the crude oil degrading Stenotrophomonas sp. B-2: chemical characterization, biological activities and environmental applications. Environ Sci Pollut Res 24(4):3769–3779. https://doi.org/10.1007/s11356-016-8064-4
Otterstedt K, Larsson C, Bill RM, Ståhlberg A, Boles Eckhard, Hohman Sn, Gustafsson L (2004) EMBO Reports 5(5): 532–537. https://doi.org/10.1038/sj.embor.7400132
Gudina EJ, Pereira JFB, Costa R, Evtuguin DV, Coutinho JAP, Teixeira JA, Rodrigues LR (2015) Novel bioemulsifier produced by a Paenibacillus strain isolated from crude oil. Microb Cell Fact. https://doi.org/10.1186/s12934-015-0197-5
Ortega-de la Rosa ND, Vázquez-Vázquez JL, Huerta-Ochoa S, Gimeno M, Gutiérrez-Rojas M (2018) Stable bioemulsifiers are produced by Acinetobacter bouvetii UAM25 growing in different carbon sources. Bioproc Biosyst Eng 41:859–869. https://doi.org/10.1007/s00449-018-1920-5
Khopade A, Biao R, Liu X, Mahadik K, Zhang L, Kokare C (2012) Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination 285:198–204. https://doi.org/10.1016/j.desal.2011.10.002
van Gulik WM, Heijnen JJ (1995) A metabolic network stoichiometry analysis of microbial growth and product formation. Biotechnol Bioeng 48:681–698. https://doi.org/10.1002/bit.260480617
Patil JR, Chopade BA (2001) Studies on bioemulsifier production by Acinetobacter strains isolated from healthy human skin. J Appl Microbiol 91:290–298
Velioglu Z, Urek RO (2015) Optimization of cultural conditions for biosurfactant production by Pleurotus djamor in solid state fermentation. J Biosci Bioeng 120(5):526–531. https://doi.org/10.1016/j.jbiosc.2015.03.007
Randhawa M (2019) Robbinsville , NJ,US. Topical composition containing glycerin and yeast extract (US Patent; Patent No- 10,206,870, B2, Date- Feb, 19, 2019)
Kaplan N, Zosim Z, Rosenberg E (1987) Reconstitution of emulsifying activity of Acinetobacter calcoaceticus BD4 Emulsan by using pure polysaccharide and protein. Appl Environ Microbiol 53(2):440–446
Zhao YH, Chen LY, Tian ZJ, Sun Y, Liu JB, Huang L (2016) Characterization and application of a novel bioemulsifier in crude oil degradation by Acinetobacter beijerinckii ZRS. J Basic Microbiol 56:184–195. https://doi.org/10.1002/jobm.201500487
Pines O, Bayer EA, Gutnick DL (1983) Localization of emulsan-like polymer associated with the cell surface of Acinetobacter calcoaceticus. J Bacteriol 154(2):893–905