Production, partial purification and characterization of a proteoglycan bioemulsifier from an oleaginous yeast

Bioprocess and Biosystems Engineering - Tập 43 - Trang 1747-1759 - 2020
Moumita Bhaumik1, Gunaseelan Dhanarajan1, Jayita Chopra1, RaviRanjan Kumar1, Chinmay Hazra1, Ramkrishna Sen1
1Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, India

Tóm tắt

In this study, Meyerozyma caribbica, an indigenously isolated oleaginous yeast, produced in media containing glucose a bioemulsifier that was partially characterized as a proteoglycan based on preliminary analysis. Optimization of carbon:nitrogen (C:N) ratio revealed 30:1 as the suitable ratio for enhanced production. Apart from higher emulsification activity (E24: 70–80%), this molecule showed strong emulsion stability over a wide range of pH (2.0–9.0), salinity (0.05%—10%, w/v) and temperature (− 80 °C to + 50 °C). The current study emphasizes on the determination of critical media parameters for improved and stable bioemulsifier production coupled with partial characterization and identification of the molecule. Thus, a proteoglycan-based bioemulsifier with such a stable emulsifying property can serve as a versatile and potential component in food, cosmetics and pharmaceutical formulations.

Tài liệu tham khảo

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