Production of ethanol from hemicellulose fraction of cocksfoot grass using pichia stipitis
Tóm tắt
In this study, cocksfoot grass (Dactylis glomerata), an abundant lignocellulosic biomass was pretreated using different operational parameters using wet explosion (WEx) pretreatment for accessing the bioethanol potential of the hemicellulose fraction. Utilization of the hemicellulose liquid hydrolysate to ethanol is essential for economically feasible cellulosic ethanol processes. Fermentation of the separated hemicellulose liquid hydrolysates obtained after the WEx pretreatment was done by Pichia stipitis CBS 6054 (Scheffersomyces stipitis). The fermentation of the WEx liquid hydrolysate from the pretreatment at higher severity (180°C, 15 min, 87 psi oxygen and 190°C, 15 min, 0.2% sulfuric acid) was fully inhibited probable by the presence of higher concentrations of inhibitory compounds such as furfural, HMF and acetic acid. The ethanol yield among other WEx conditions was in the range of 89 to 158 mL/kg DM, with the highest yield (92% of theoretical maximum value) found for the lower pretreatment severity at 160°C, 15 min, 87 psi oxygen. Our findings from this present study demonstrated that the release of hemicellulose sugars in the liquid hydrolysate is maximal when a lower pretreatment severity is applied. This is evident as the highest ethanol yields were found under the pretreatment conditions at lower severity.
Tài liệu tham khảo
Taherzadeh MJ, Karimi K: Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: a review. Int J Sci. 2008, 9: 1621-1651.
Chen H, Qiu W: Key technologies for bioethanol production from lignocellulose. Biotechnol Adv. 2010, 28: 556-562.
Petersson A, Thomsen MH, Hauggaard-Nielsen H, Thomsen AB: Potential bioethanol and biogas production using lignocellulosic biomass from winter rye, oilseed rape and faba bean. Biomass Bioenergy. 2007, 31: 812-819. 10.1016/j.biombioe.2007.06.001.
Kristensen JB, Thygesen LG, Felby C, Jørgensen H, Elder T: Cell-wall structure changes in wheat straw pretreated for bioethanol production. Biotechnol for Biofuels. 2008, 1: 1-9. 10.1186/1754-6834-1-1.
Jeffries TW: Engineering yeasts for xylose metabolism. Curr Opin Biotechnol. 2006, 17: 320-326. 10.1016/j.copbio.2006.05.008.
Sassner P, Mårtensson CG, Galbe M, Zacchi G: Steam pretreatment of H2SO4–impregnated Salix for the production of bioethanol. Bioresource Technol. 2008, 99: 137-145. 10.1016/j.biortech.2006.11.039.
Galbe M, Zacchi G: A review of the production of ethanol from softwood. Appl Microbiol Biotechnol. 2002, 59: 618-628. 10.1007/s00253-002-1058-9.
Hendriks ATWM, Zeeman G: Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresource Technol. 2009, 100: 10-18. 10.1016/j.biortech.2008.05.027.
Agbogbo FK, Wenger KS: Production of ethanol from corn Stover hemicellulose hydrolysate using pichia stipitis. Ind Microbiol Biotechnol. 2007, 34: 723-727. 10.1007/s10295-007-0247-z.
Zhu JJ, Yong Q, Xu Y, Chen SX, Yu SY: Adaptation fermentation of pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate. Nat Sci. 2009, 1: 47-54.
Taniguchi M, Tohma T, Itaya T, Fujii M: Ethanol production from a mixture of glucose and xylose by co-culture of pichia stipites and a respiratory-deficient mutant of saccharomyces cerevisiae. Jour Ferment Bioeng. 1997, 83: 364-370. 10.1016/S0922-338X(97)80143-2.
Moniruzzaman M: Alcohol fermentation of enzymatic hydrolysate of exploded rice straw by pichia stipitis. World Jour of Microbiol Biotechnol. 1995, 11: 646-648. 10.1007/BF00361008.
Agbogbo FK, Coward-Kelly G: Cellulosic ethanol production using the naturally occurring xylose-fermenting yeast, pichia stipitis. Biotechnol Lett. 2008, 30: 1515-1524. 10.1007/s10529-008-9728-z.
Parekh SR, Parekh RS, Wayman M: Fermentation of xylose and cellobiose by pichia stipitis and brettanomyces clausenii. Appl Biochem Biotechnol. 1988, 18: 325-338. 10.1007/BF02930836.
Shi X-Q, Jeffries TW: Anaerobic growth and improved fermentation of pichia stipitis bearing a URA1 gene from saccharomyces cerevisiae. Appl Microbiol Biotechnol. 1998, 50: 339-345. 10.1007/s002530051301.
Delgenes JP, Moletta R, Navarro JM: The effect of aeration on D-xylose fermentation by P. Tannophilus, P. Stipitis, K. Marxianus and C. Shehatae. Biotechnol Lett. 1986, 8: 897-900. 10.1007/BF01078656.
du Preez JC, Bosch M, Prior BA: Xylose fermentation by Candida shehatae and pichia stipitis: effects of pH, temperature and substrate concentration. Enzyme and Microb Technol. 1986, 8: 360-364. 10.1016/0141-0229(86)90136-5.
Zhong C, Lau MW, Balan V, Dale BE, Yuan YJ: Optimization of enzymatic hydrolysis and ethanol fermentation from AFEX-treated rice straw. Appl Microbiol Biotechnol. 2009, 84: 667-676. 10.1007/s00253-009-2001-0.
Roberto IC, Lacis LS, Barbosa MFS, de Mancilha IM: Utilization of sugarcane bagasse hemicellulosic hydrolysate by pichia stipitis for the production of ethanol. Process Biochem. 1991, 26: 15-21. 10.1016/0032-9592(91)80003-8.
Palmqvist E, Hahn-Hägerdal B: Fermentation of lignocellulosic hydrolysates II: inhibitors and mechanisms of inhibition. Bioresource Technol. 2000, 74: 25-33. 10.1016/S0960-8524(99)00161-3.
Bellido C, Bolado S, Coca M, Lucas S, Gonzalez-Benito G, Garcia-Cubero MT: Effect of inhibitors formed during wheat straw pretreatment on ethanol fermentation by pichia stipitis. Bioresource Technol. 2011, 102: 10868-10874. 10.1016/j.biortech.2011.08.128.
Scordia D, Cosentino SL, Jeffries TW: Second generation bioethanol production from saccharun spontaneum L. ssp. Aegyptiacum (wild.) hack. Bioresource Technol. 2010, 101: 5358-5365. 10.1016/j.biortech.2010.02.036.
Cantarella M, Cantarella L, Gallifuoco A, Spera A, Alfan F: Comparison of different detoxification methods for steam-exploded poplar wood as a substrate for the bioproduction of ethanol in SHF and SSF. Process Biochem. 2004, 39: 1533-1542. 10.1016/S0032-9592(03)00285-1.
Chandel AK, Kapoor RK, Singh A, Kuhad RC: Detoxification of sugarcane bagasse hydrolysate improves ethanol production by Candida shehatae NCIM 3501. Bioresource Technol. 2007, 98: 1947-1950. 10.1016/j.biortech.2006.07.047.
Ferrari MD, Neirotti E, Albornoz C, Saucedo E: Ethanol production from eucalyptus wood hemicellulose hydrolysate by pichia stipitis. Biotechnol and Bioeng. 1992, 40: 753-759. 10.1002/bit.260400702.
Rana D, Rana V, Ahring BK: Producing high sugar concentrations from loblolly pine using wet explosion pretreatment. Bioresource Technol. 2012, 121: 61-67.
Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D: Determination of structural carbohydrates and lignin in biomass. Laboratory Analytical Procedure. 2008, http://www.nrel.gov/biomass/pdfs/42618.pdf,
American Public Health Association (APHA), American Water Works Association (AWWA), and Water Environment Federation (WAE): Standard Methods for the Examination of Water and Waste Water: physical and aggregate properties. Edited by: Greenberg AE, Clesceri LS, Trussel RR. 1992, USA: Washington D. C, 18
Hatzis C, Riley C, Philippidis GP: Detailed material balance and ethanol yield calculations for biomass-to-ethanol conversion process. Appl Biochem Biotechnol. 1996, 96: 0273-2289.