Primordial follicle survival of goat ovarian tissue after vitrification and transplantation on chorioallanthoic membrane

Shofwal Widad1, Detty Siti Nurdiati2, Sarrah Ayuandari2, Kuky Cahya Hamurajib1, Muhammad Dimas Reza Rahmana3, Nurulita Ainun Alma1, Agung Dewanto1
1Fertility and Reproduction Endocrinology Division, Department of Obstetrics and Gynecology, Universitas Gadjah Mada–Dr. Sardjito Hospital, Yogyakarta, Indonesia
2Department of Obstetrics and Gynecology, Universitas Gadjah Mada–Dr. Sardjito Hospital, Yogyakarta, Indonesia
3Biomedical Science Postgraduate Program, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

Tóm tắt

Abstract Background Ovarian tissue vitrification is an alternative fertility preservation procedure for young female patients prior to gonadotoxic treatment. Primordial follicle loss might be a potential issue for vitrification and transplantation procedures. This study aimed to evaluate primordial follicle density and deoxyribonucleic acid (DNA) fragmentation in each stage of the preservation procedure of goat ovarian tissue. Follicle density and DNA fragmentation were examined microscopically after staining with hematoxylin eosin and TUNEL assay, respectively. Both parameters were compared between fresh, fresh-transplanted, vitrification, and vitrification-transplanted groups. Results A significant decrease was observed in the primordial follicle proportion after vitrification and transplantation compared to the primordial follicle proportion in the fresh group (88.09% vs 52.42%, p < 0.05, 95% CI 11.54, 66.94). There was no significant difference in DNA fragmentations of primordial follicles between each group (p > 0.05). Conclusions The vitrification and transplantation process of goat ovarian strips could cause the primordial follicles loss and DNA damage of the follicles. However, primordial follicles loss and DNA damage were not significantly different in each procedure.

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Tài liệu tham khảo

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