Priming the immune system of Penaeid shrimp by bacterial <scp>HSP</scp>70 (DnaK)

Journal of Fish Diseases - Tập 39 Số 5 - Trang 555-564 - 2016
L H Phuoc1, Bing Hu2, Mathieu Wille2, N T Hien1, V H Phuong1, Nguyễn Thị Tình1, N H Loc1, Patrick Sorgeloos2, Peter Bossier2
1Research Institute for Aquaculture No2; Ho Chi Minh City Vietnam
2Laboratory for Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium.

Tóm tắt

AbstractThis study was conducted to test the effect of DnaK on priming immune responses in Penaeid shrimp. Juvenile‐specific pathogen‐free (SPF) P. vannamei shrimp were injected with 0.05 μg recombinant DnaK. One hour post‐DnaK priming, a non‐lethal dose of Vibrio campbellii (10CFU shrimp−1) was injected. Other treatments include only DnaK or V. campbellii injection or control with blank inocula. The haemolymph of three shrimp from each treatment was collected at 1.5, 6, 9 and 12 h post‐DnaK priming (hpp). It was verified that injection with DnaK and V. campbellii challenge affected the transcription of 3 immune genes, transglutaminase‐1 (TGase‐1), prophenoloxidase‐2 (proPO‐2) and endogenous HSP70 (lvHSP70). In P. monodon, shrimp were first injected with DnaK at a dose of 10 μg shrimp−1 and one hour later with 10CFU of V. harveyi (BB120) shrimp−1. Shrimp injected with DnaK showed a significant increase in proPO expression compared to the control (P < 0.05). Yet a double injection (DnaK and Vibrio) seemed to cause an antagonistic response at the level of expression, which was not equalled at the level of PO activity. Those results suggest that DnaK is able to modulate immune responses in P. vannamei and P. monodon.

Từ khóa


Tài liệu tham khảo

10.1016/j.dci.2008.09.003

10.1016/j.gene.2013.04.044

10.1016/j.fsi.2010.07.011

10.1016/j.fsi.2012.10.025

CereniusL. JiravanichpaisalP. LiuH.P.&SoderhallI.(2010)Crustaceans Immunity in: Link.Springer.com Advances in Experimental Medicine and Biology. Springer US Boston MA pp. 239–259.

10.1016/j.dci.2010.09.002

10.1016/j.dci.2009.07.006

10.1016/j.aquaculture.2012.04.042

10.1371/journal.pbio.0040229

10.1016/j.fsi.2012.01.018

10.1016/j.molimm.2006.02.018

10.1016/j.fsi.2013.03.368

10.1016/j.aquaculture.2013.10.008

10.1182/blood-2010-11-320614

10.1128/JVI.01973-10

10.1016/j.molimm.2013.03.023

10.1006/meth.2001.1262

10.1016/j.molimm.2007.09.016

10.1016/j.chom.2012.09.006

10.1098/rspb.2003.2511

10.1016/S0162-3109(00)00227-7

10.1016/j.chom.2011.04.006

10.1007/s00430-007-0055-0

10.1111/j.1462-2920.2008.01692.x

10.1016/j.aquaculture.2009.02.004

10.1016/j.cell.2006.02.012

10.1111/j.1749-6632.2009.05375.x

10.1016/j.fsi.2009.09.005

10.1016/j.cub.2006.04.047

10.3109/10408449309104074

10.1016/0145-305X(91)90018-T

10.1038/nri749

10.1016/j.coi.2004.05.007

10.1007/s12192-008-0011-y

10.1111/j.1365-2761.2009.01046.x

10.4049/jimmunol.172.9.5277

10.1046/j.1365-2567.2003.01725.x

10.1152/ajpcell.00364.2003

10.1016/j.dci.2007.10.010

10.1111/j.1365-2109.2010.02602.x

10.1126/science.1116887

10.1016/j.fsi.2008.10.003

10.1016/j.fsi.2009.09.006

10.1016/j.jip.2009.12.006