Primary human osteoblasts grow into porous tantalum and maintain an osteoblastic phenotype

Journal of Biomedical Materials Research - Part A - Tập 84A Số 3 - Trang 691-701 - 2008
Katie J. Welldon1, Gerald J. Atkins1, Donald W. Howie1, David M. Findlay1
1Department of Orthopaedics and Trauma, University of Adelaide, Adelaide, South Australia, Australia.

Tóm tắt

AbstractPorous tantalum (Ta) has found application in orthopedics, although the interaction of human osteoblasts (HOB) with this material has not been reported. The aim of this study was to investigate the interaction of primary HOB with porous tantalum, using 5‐mm thick discs of porous tantalum. Comparison was made with discs of solid tantalum and tissue culture plastic. Confocal microscopy was used to investigate the attachment and growth of cells on porous Ta, and showed that HOB attached successfully to the metal “trabeculae,” underwent extensive cell division, and penetrated into the Ta pores. The maturation of HOB on porous Ta was determined in terms of cell expression of the osteoblast phenotypic markers, STRO‐1, and alkaline phosphatase. Despite some donor‐dependent variation in STRO‐1/AlkPhos expression, growth of cells grown on porous Ta either promoted, or did not impede, the maturation of HOB. In addition, the expression of key osteoblastic genes was investigated after 14 days of culture. The relative levels of mRNA encoding osteocalcin, osteopontin and receptor activator of NFκB ligand (RANKL) was not different between porous or solid Ta or plastic, although these genes were expressed differently by cells of different donors. However, bone sialoprotein and type I collagen mRNA species showed a decreased expression on porous Ta compared with expression on plastic. No substrate‐dependent differences were seen in the extent of in vitro mineralization by HOB. These results indicate that porous Ta is a good substrate for the attachment, growth, and differentiated function of HOB. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008

Từ khóa


Tài liệu tham khảo

Cohen R, 2002, A porous tantalum trabecular metal: Basic science, Am J Orthop, 31, 216

10.1002/jbm.b.30229

10.1016/j.biomaterials.2006.04.041

10.1302/0301-620X.81B5.9283

10.1016/S0883-5403(99)90062-1

10.1002/1097-4636(20001215)52:4<631::AID-JBM7>3.0.CO;2-6

10.1007/s00264-005-0008-4

Stiehl JB, 2005, Trabecular metal in hip reconstructive surgery, Orthopedics, 28, 662, 10.3928/0147-7447-20050701-13

10.1016/j.jhsa.2005.08.008

10.4263/jorthoptic.31.219

10.1016/j.arth.2004.09.029

10.1016/S0883-5403(03)00290-0

10.1016/j.arth.2005.03.020

10.1111/j.1601-6343.2005.00329.x

10.1016/j.arth.2005.01.023

10.1016/j.biomaterials.2003.09.005

10.1242/dev.117.4.1183

10.1359/jbmr.1999.14.1.47

Lyons AB, 2001, Flow Cytometric Analysis of Cell Division History Using Dilution of Carboxyfluorescein Diacetate Succinimidyl Ester, a Stably Integrated Fluorescent Probe

10.1002/jcp.20255

10.1359/jbmr.2000.15.4.640

10.22203/eCM.v011a02

10.1080/14653240310003585

10.1089/152581603322448277

10.1016/j.biomaterials.2006.05.053

10.1016/j.biomaterials.2004.10.033

10.1016/j.bone.2005.11.027

Lian JB, 1996, Osteoporosis, 23

Aubin JE, 1996, The Osteoblast Lineage—Embryologic Origins and the Differentiation Sequence

10.1002/jbm.820250603

10.1002/jbm.820280813

10.1034/j.1600-0501.2002.130608.x

10.1007/s10856-004-5680-1

Thông tin
Thông tin xuất bản

Journal of Biomedical Materials Research - Part A

Tập 84A Số 3

691-701

Thông tin tác giả