Prevention by silymarin of membrane alterations in acute CCI4 liver damage

Journal of Applied Toxicology - Tập 10 Số 4 - Trang 275-279 - 1990
Pablo Muriel1, Marisabel Mourelle1
1Departamento de Farmacologia y Toxicologia. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Apartado Postal 14–740, 07000 Mexico, D.F

Tóm tắt

AbstractThe effect of silymarin on liver lipid peroxidation and membrane lipid alterations induced by an acute dose of CCI4 was studied. Four groups of animals were treated with CCI4, CCI4 + silymarin, silymarin and its vehicles. CCI4 was given orally (0.4 g 100 g−1 body wt.) and silymarin was administered i.p. All animals were sacrificed 24 h after the treatments. Liver lipid peroxidation was measured and plasma membranes were isolated. Alkaline phosphatase (AP) and gamma‐glutamyl transpeptidase (GGTP) were measured in plasma membranes. Membrane lipids were extracted and then analysed by thin‐layer chromatography by measuring the phosphorus of the phospholipids in each spot. Liver lipid peroxidation was increased about three times in the group receiving CCI4 only. Silymarin cotreatment prevented this increase. Phosphatidylethanolamine (PEA) decreased, while phosphatidylinositol (PI) increased in the plasma membranes isolated from the CCI4‐treated group. Animals that received CCI4 + silymarin showed no decrease in PEA content. A partial prevention of the decrease in phosphatidylinositol content was also observed in plasma membranes of animals treated with silymarin in addition to CCI4. CCI4 decreased gamma‐glutamyl transpeptidase (GGTP) and alkaline phosphatase (AP) membrane activities. Silymarin cotreatment prevented the AP (completely) and the GGTP (partially) falls caused by CCI4. Silymarin by itself increased AP membrane activity.A significant relationship between the membrane content of phosphatidylethanolamine (PEA) and the AP activity was observed in plasma membranes of treated animals and in normal liver membranes enriched with PEA. These results indicate that silymarin can protect against the alterations induced by CCI4 on the liver plasma membrane through its antioxidant properties by modifying the plasma membrane phospholipid content.

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Tài liệu tham khảo

McLean A. E. M., 1965, Cellular necrosis in the liver induced and modified by drugs, Int. Rev. Exp. Pathol., 4, 127

10.1002/hep.1840040124

10.1254/jjp.23.627

10.1254/jjp.23.653

10.1254/jjp.23.639

10.1254/jjp.23.645

10.1016/0041-008X(84)90187-X

10.3109/10408447309082019

10.1016/0026-0495(67)90050-9

10.1016/S0168-8278(86)80036-8

10.1016/0014-4800(85)90073-5

10.1002/hep.1840030608

Popper H., 1977, Membrane Alterations as Basis of Liver Injury.

Ramellini G., 1974, Stabilization of isolated rat liver plasma membranes by treatment in vitro with silymarin, Arzneim‐Forsch., 24, 806

Porcellati G., 1976, Symposium on the Pharmacodynamics of Silymarin, 84

10.1007/BF02533425

10.1083/jcb.8.2.413

10.1016/S0021-9258(18)64849-5

10.1016/0076-6879(66)08014-5

10.1016/S0021-9258(19)50907-3

Skipski P. V., 1969, Thin‐layer chromatography of lipids, Methods Enzymol., 14, 330

10.1016/0003-2697(76)90527-3

Berger L., 1963, Standard Methods of Clinical Chemistry

10.1016/0014-5793(72)80075-9

Chapman D., 1968, Biological Membranes: Physical Fact and Function, 125

10.1073/pnas.77.1.257

Coodley E. L., 1969, Enzyme diagnosis in hepatobiliary disease, Am. J. Gastroenterol., 52, 189

10.1016/0014-4800(68)90041-5

Desplaces A., 1975, The effects of silymarin on experimental phalloidine poisoning, Arzneim.‐Forsch., 25, 89

Thông tin
Thông tin xuất bản

Journal of Applied Toxicology

Tập 10 Số 4

275-279

Thông tin tác giả