Prevalence and Subtype Distribution of Blastocystis sp. in Senegalese School Children

Microorganisms - Tập 8 Số 9 - Trang 1408
Salma M. Khaled1, Nausicaa Gantois1, Amadou T. Ly2, Simon Senghor2, Gaël Even3,4, Ellena Dautel1, Romane Dejager1, Manasi Sawant1, Martha Baydoun1, Sadia Benamrouz-Vanneste1,5, Magali Chabé1, Seynabou Ndiaye6, Anne‐Marie Schacht1,2, Gabriela Certad1,7, Gilles Riveau1,2, Éric Viscogliosi1
1CIIL - Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (1 Rue du Professeur Calmette - Lille Cedex - 59019 - BP 245 - France)
2Centre de Recherche Biomédicale - Espoir pour la santé (EPLS) (EPLS Sénégal Maison des Programmes 263, route de la corniche. BP 226 Saint-Louis, Sénégal tel: (221) 33 961 03 77 fax: (221) 33 961 03 77 - Senegal)
3Gènes Diffusion [Douai] (3595 Route de Tournai, 59501 Douai CEDEX - France)
4PEGASE-Biosciences - Plateforme d'expertises génomiques appliquées aux sciences expérimentales [Lille] (2ème étage - Bâtiment Guerin, Institut Pasteur de Lille - 1 rue du professeur Calmette, 59000 Lille - France)
5ICL - Institut Catholique de Lille (60, boulevard Vauban / 59800 LILLE - France)
6MSAS - Ministère de la Santé et de l'Action sociale (Sénégal) (Dakar - Senegal)
7GHICL - Groupement des Hôpitaux de l'Institut Catholique de Lille (France)

Tóm tắt

Blastocystis sp. is an enteric protozoan that frequently colonizes humans and many animals. Despite impacting on human health, data on the prevalence and subtype (ST) distribution of Blastocystis sp. remain sparse in Africa. Accordingly, we performed the first multicenter and largest epidemiological survey ever conducted on Blastocystis sp. for this continent. A total of 731 stool samples collected from healthy school children living in 10 villages of the northwestern region of Senegal were tested for the presence of Blastocystis sp. by real-time polymerase chain reaction followed by subtyping of positive samples. Considerable variation in prevalence between villages (51.7 to 100%) was evident with the overall prevalence being 80.4%. Mixed infections were identified in 23% of positive individuals. Among 453 school children with a single infection, ST2 was predominant, followed by ST1, ST3, ST7, ST10, and ST14; this is the first report of ST10 and ST14 in humans. Genetic polymorphisms were evident at the intra-ST level with the identification of numerous ST1 to ST3 genotypes. ST1 showed the greatest intra-ST diversity followed by ST2 and ST3. The prevalence and distribution of STs and genotypes varied among target villages, pointing to several potential infection sources, including human-to-human, zoonotic, and waterborne transmission.

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Microorganisms

Tập 8 Số 9

1408

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