Pressurized liquid extraction followed by LC‐ESI/MS for analysis of four chromones in Radix Saposhnikoviae

Journal of Separation Science - Tập 33 Số 17-18 - Trang 2881-2887 - 2010
Wei Li1,2,3, Zi Wang1,3, Li Chen2, Jing Zhang1, Li‐Kun Han4, Jingang Hou1, Yinan Zheng1
1College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, P. R. China
2Norman Bethune College of Medicine, Jilin University, Changchun, P. R. China
3The first two authors contributed equally to this work and therefore should be considered co-first authors.
4Beauty Care Laboratory, Kracie Home Products Ltd., Yokohama-City, Japan

Tóm tắt

AbstractAn efficient pressurized liquid extraction (PLE) technique was employed in extracting chromones from the roots of Saposhnikovia divaricata (Radix Saposhnikoviae). Chromones were quantified and analyzed by LC‐ESI/MS. The PLE procedure was optimized, validated and compared with the other conventional extraction techniques. PLE gained the best result due to the highest extraction efficiency within the shortest extraction time. The optimal conditions of PLE were employing 50% ethanol as the extraction solvent at a temperature of 140°C and an extraction pressure of 1500 psi, using one extraction cycle with a static extraction time of 8 min. A good LC separation was achieved using a Hypersil ODS2 column and methanol/water as the mobile phase at a flow rate of 0.8 mL/min. MS coupling with an ESI interface in the positive ion mode was used as the detection technique. This is the first report on combining PLE with LC‐ESI/MS for the extraction and quantification of chromones in Radix Saposhnikoviae. The elaborated PLE method also provided a good alternative for the chromone extraction from other plant substances.

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Thông tin
Thông tin xuất bản

Journal of Separation Science

Tập 33 Số 17-18

2881-2887

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