Preservation of Membranes in Anhydrobiotic Organisms: The Role of Trehalose

American Association for the Advancement of Science (AAAS) - Tập 223 Số 4637 - Trang 701-703 - 1984
John H. Crowe1, Lois M. Crowe1, D. Chapman2
1Department of Zoology, University of California, Davis 95616, and Department of Biochemistry and Chemistry, Royal Free Hospital Medical School, London NW3, United Kingdom
2Department of Biochemistry and Chemistry, Royal Free Hospital Medical School

Tóm tắt

Trehalose is a nonreducing disaccharide of glucose commonly found at high concentrations in anhydrobiotic organisms. In the presence of trehalose, dry dipalmitoyl phosphatidylcholine (DPPC) had a transition temperature similar to that of the fully hydrated lipid, whereas DPPC dried without trehalose had a transition temperature about 30 degrees Kelvin higher. Results obtained with infrared spectroscopy indicate that trehalose and DPPC interact by hydrogen bonding between the OH groups in the carbohydrate and the polar head groups of DPPC. These and previous results show that this hydrogen bonding alters the spacing of the polar head groups and may thereby decrease van der Waals interactions in the hydrocarbon chains of the DPPC. This interaction between trehalose and DPPC is specific to trehalose. Hence this specificity may be an important factor in the ability of this molecule to stabilize dry membranes in anhydrobiotic organisms.

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Tài liệu tham khảo

Amey R. Biomembrane Structure nd Function (1984).

CADENHEAD, D.A., OBSERVATIONS AND IMPLICATIONS OF GLYCEROL-MONOMOLECULAR FILM INTERACTIONS, BIOCHIMICA ET BIOPHYSICA ACTA 176: 849 (1969).

CHAPMAN, D, CHEM PHYS LIPIDS 1: 445 (1967).

CLEGG, J.S., ORIGIN OF TREHALOSE AND ITS SIGNIFICANCE DURING FORMATION OF ENCYSTED DORMANT EMBRYOS OF ARTEMIA SALINA, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY 14: 135 (1965).

CLEGG, J.S., CONTROL EMERGENCE AND METABOLISM BY EXTERNAL OSMOTIC PRESSURE AND ROLE OF FREE GLYCEROL IN DEVELOPING CYSTS OF ARTEMIA SALINA, JOURNAL OF EXPERIMENTAL BIOLOGY 41: 879 (1964).

CLEGG, J.S., TREHALOSE IN CELLULAR SLIME MOULD DICTYOSTELIUM-MUCOROIDES, NATURE 192: 1077 (1961).

Crowe J. H. Anhydrobiosis (1973).

CROWE, J.H., PRESERVATION OF STRUCTURAL AND FUNCTIONAL-ACTIVITY IN LYOPHILIZED SARCOPLASMIC-RETICULUM, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 220: 477 (1983).

Crowe, J. H., Biochimica et Biophysica Acta: Biomembranes 769: 151 (1984).

Crowe J. H. Biological Membranes in press.

Crowe J. H. Dry Biological Systems (1978).

CROWE, L.M., HYDRATION-DEPENDENT HEXAGONAL PHASE LIPID IN A BIOLOGICAL MEMBRANE, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 217: 582 (1982).

Crowe, L. M., Biochimica et Biophysica Acta: Biomembranes 769: 151 (1984).

CROWE L.M. unpublished data.

ELIASZ, A.W., PHOSPHOLIPID PHASE-TRANSITIONS EFFECTS OF NORMAL-ALCOHOLS, NORMAL-MONOCARBOXYLIC ACIDS, PHENYLALKYL ALCOHOLS AND QUATERNARY AMMONIUM-COMPOUNDS, BIOCHIMICA ET BIOPHYSICA ACTA 448: 220 (1976).

FOOKSON, J.E., STRUCTURAL DIFFERENCES AMONG PHOSPHATIDYLCHOLINE, PHOSPHATIDYLETHANOLAMINE, AND MIXED PHOSPHATIDYLCHOLINE-PHOSPHATIDYLETHANOLAMINE MULTILAYERS - INFRARED-ABSORPTION STUDY, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 189: 195 (1978).

HARDING, T.S., SUGAR 25: 476 (1923).

HAUSER, H, PREFERRED CONFORMATION AND MOLECULAR PACKING OF PHOSPHATIDYLETHANOLAMINE AND PHOSPHATIDYLCHOLINE, BIOCHIMICA ET BIOPHYSICA ACTA 650: 21 (1981).

MADIN, KAC, ANHYDROBIOSIS IN NEMATODES - CARBOHYDRATE AND LIPID-METABOLISM DURING DEHYDRATION, JOURNAL OF EXPERIMENTAL ZOOLOGY 193: 335 (1975).

MAGGIO, B, INTERACTIONS OF WATER-SOLUBLE FUSOGENS WITH PHOSPHOLIPIDS IN MONOLAYERS, FEBS LETTERS 94: 301 (1978).

MCDANIEL, R.V., NON-ELECTROLYTE SUBSTITUTION FOR WATER IN PHOSPHATIDYLCHOLINE BILAYERS, BIOCHIMICA ET BIOPHYSICA ACTA 731: 97 (1983).

PAYEN, R, VARIATIONS DES TENEURS EN GLYCOGENE ET EN TREHALOSE PENDANT LE SECHAGE DE LA LEVURE, CANADIAN JOURNAL OF RESEARCH SECTION B-CHEMICAL SCIENCES 27: 749 (1949).

10.1126/science.130.3385.1343

SUURKUUSK, J, CALORIMETRIC AND FLUORESCENT-PROBE STUDY OF GEL-LIQUID CRYSTALLINE PHASE-TRANSITION IN SMALL, SINGLE-LAMELLAR DIPALMITOYLPHOSPHATIDYLCHOLINE VESICLES, BIOCHEMISTRY 15: 1393 (1976).

WOMERSLEY, C, BIOCHEMICAL AND PHYSIOLOGICAL-ASPECTS OF ANHYDROBIOSIS, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 70: 669 (1981).

WUNDERLICH, F, THERMOTROPIC FLUID -] ORDERED DISCONTINUOUS PHASE SEPARATION IN MICROSOMAL LIPIDS OF TETRAHYMENA - X-RAY-DIFFRACTION STUDY, BIOCHEMISTRY 17: 2005 (1978).

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American Association for the Advancement of Science (AAAS)

Tập 223 Số 4637

701-703

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