Presence of mcr-1-positive Enterobacteriaceae in retail chicken meat but not in humans in the Netherlands since 2009

Eurosurveillance - Tập 21 Số 9 - 2016
Maurine A. Leverstein‐van Hall1,2, Pepijn Huizinga3, Marc J. M. Bonten4,2, Lieuwe D. J. Bos5, Katrien De Bruyne6, Alexander W. Friedrich7, John W. A. Rossen7, Paul H. M. Savelkoul8,9, Jan Kluytmans2,3
1Amphia Academy Infectious Disease Foundation, Amphia Hospital, Breda, The Netherlands
2Julius Centre for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht, The Netherlands
3Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, the Netherlands
4Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
5Microbiome Ltd., Amsterdam, The Netherlands
6Applied Maths NV, Sint-Martens-Latem, Belgium
7Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands
8Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands
9Department of Medical Microbiology, Maastricht University Medical Centre, Maastricht, The Netherlands

Tóm tắt

Recently, the plasmid-mediated colistin resistance gene mcr-1 was found in Enterobacteriaceae from humans, pigs and retail meat in China. Several reports have documented global presence of the gene in Enterobacteriaceae from humans, food animals and food since. We screened several well-characterised strain collections of Enterobacteriaceae, obtained from retail chicken meat and hospitalised patients in the Netherlands between 2009 and 2015, for presence of colistin resistance and the mcr-1 gene. A total of 2,471 Enterobacteriaceae isolates, from surveys in retail chicken meat (196 isolates), prevalence surveys in hospitalised patients (1,247 isolates), clinical cultures (813 isolates) and outbreaks in healthcare settings (215 isolates), were analysed. The mcr-1 gene was identified in three (1.5%) of 196 extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates from retail chicken meat samples in 2009 and 2014. Two isolates were obtained from the same batch of meat samples, most likely representing contamination from a common source. No mcr-1-positive isolates were identified among 2,275 human isolates tested. All mcr-1-positive isolates were colistin-resistant (minimum inhibitory concentration (MIC) > 2 mg/L). Our findings indicate that mcr-1-based colistin-resistance currently poses no threat to healthcare in the Netherlands. They indicate however that continued monitoring of colistin resistance and its underlying mechanisms in humans, livestock and food is needed.

Từ khóa


Tài liệu tham khảo

Doi, 2015, Carbapenemase-producing Enterobacteriaceae., Semin Respir Crit Care Med, 36, 74, 10.1055/s-0035-1544208

Liu, 2016, Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study., Lancet Infect Dis, 16, 161, 10.1016/S1473-3099(15)00424-7

Hasman, 2015, Detection of mcr-1 encoding plasmid-mediated colistin-resistant Escherichia coli isolates from human bloodstream infection and imported chicken meat, Denmark 2015., Euro Surveill, 20, 30085, 10.2807/1560-7917.ES.2015.20.49.30085

Arcilla, 2016, Dissemination of the mcr-1 colistin resistance gene., Lancet Infect Dis, 16, 147, 10.1016/S1473-3099(15)00541-1

Olaitan, 2016, Dissemination of the mcr-1 colistin resistance gene., Lancet Infect Dis, 16, 147, 10.1016/S1473-3099(15)00540-X

Falgenhauer L, Waezsada SE, Yao Y, Imirzalioglu C, Käsbohrer A, Roesler U, et al.; RESET consortium. Colistin resistance gene mcr-1 in extended-spectrum β-lactamase-producing and carbapenemase-producing Gram-negative bacteria in Germany. Lancet Infect Dis. 2016.

Malhotra-Kumar S, Xavier BB, Das AJ, Lammens C, Butaye P, Goossens H. Colistin resistance gene mcr-1 harboured on a multidrug resistant plasmid. Lancet Infect Dis. 2016.

Malhotra-Kumar S, Xavier BB, Das AJ, Lammens C, Hoang HTT, Pham NT, et al. Colistin-resistant Escherichia coli harbouring mcr-1 isolated from food animals in Hanoi, Vietnam. Lancet Infect Dis. 2016.

Stoesser N, Mathers AJ, Moore CE, Day NPJ, Crook DW. Colistin resistance gene mcr-1 and pHNSHP45 plasmid in human isolates of Escherichia coli and Klebsiella pneumoniae. Lancet Infect Dis. 2016.

Suzuki S, Ohnishi M, Kawanishi M, Akiba M, Kuroda M. Investigation of a plasmid genome database for colistin-resistance gene mcr-1. Lancet Infect Dis. 2016.

Haenni M, Poirel L, Kieffer N, Châtre P, Saras E, Métayer V, et al. Co-occurrence of extended spectrum β lactamase and MCR-1 encoding genes on plasmids. Lancet Infect Dis. 2016.

Giani, 2015, Large nosocomial outbreak of colistin-resistant, carbapenemase-producing Klebsiella pneumoniae traced to clonal expansion of an mgrB deletion mutant., J Clin Microbiol, 53, 3341, 10.1128/JCM.01017-15

Kluytmans, 2013, Extended-spectrum β-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factors., Clin Infect Dis, 56, 478, 10.1093/cid/cis929

Overdevest, 2011, Extended-spectrum β-lactamase genes of Escherichia coli in chicken meat and humans, The Netherlands., Emerg Infect Dis, 17, 1216, 10.3201/eid1707.110209

Willemsen, 2015, Trends in extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae and ESBL genes in a Dutch teaching hospital, measured in 5 yearly point prevalence surveys (2010-2014)., PLoS One, 10, e0141765, 10.1371/journal.pone.0141765

Nederlands Trial Register. Trial ID NTR2799. Amsterdam: Nederlands Trial Register. [Accessed 25 Feb 2016]. Available from: http://www.trialregister.nl/trialreg/admin/rctview.asp?TC=2799

Zhou, 2015, Characterization of a CTX-M-15 producing Klebsiella pneumoniae outbreak strain assigned to a novel sequence type (1427)., Front Microbiol, 6, 1250, 10.3389/fmicb.2015.01250

Weterings, 2015, An outbreak of colistin-resistant Klebsiella pneumoniae carbapenemase-producing Klebsiella pneumoniae in the Netherlands (July to December 2013), with inter-institutional spread., Eur J Clin Microbiol Infect Dis, 34, 1647, 10.1007/s10096-015-2401-2

Bankevich, 2012, SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing., J Comput Biol, 19, 455, 10.1089/cmb.2012.0021

Zankari, 2012, Identification of acquired antimicrobial resistance genes., J Antimicrob Chemother, 67, 2640, 10.1093/jac/dks261

Carattoli, 2014, In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing., Antimicrob Agents Chemother, 58, 3895, 10.1128/AAC.02412-14

Langmead, 2012, Fast gapped-read alignment with Bowtie 2., Nat Methods, 9, 357, 10.1038/nmeth.1923

Li, 2009, The Sequence Alignment/Map format and SAMtools., Bioinformatics, 25, 2078, 10.1093/bioinformatics/btp352

European Committee on Antimicrobial Susceptibility Testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters. Version 5.0, 2015. Available from: http://www.eucast.org/clinical_breakpoints/

Central Veterinary Institute of Wageningen University and Research Centre. MARAN 2015. Monitoring of antimicrobial resistance and antibiotic usage in animals in The Netherlands in 2014. 2015. Available from: http://www.swab.nl/swab/cms3.nsf/uploads/4F5A0D8E6F0DD139C1257E6E0051833A/$FILE/NethmapMaran2015%20_webversie.pdf

Blaak, 2014, Detection of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli on flies at poultry farms., Appl Environ Microbiol, 80, 239, 10.1128/AEM.02616-13

Wu, 2013, Comparative analysis of ESBL-positive Escherichia coli isolates from animals and humans from the UK, The Netherlands and Germany., PLoS One, 8, e75392, 10.1371/journal.pone.0075392

Dutch Foundation of the Working Party on Antibiotic Policy (SWAB). NethMap 2015. Consumption of antimicrobial agents and antimicrobial resistance among medically important bacteria in The Netherlands in 2014. Bilthoven: RIVM. 2015. Available from: http://www.swab.nl/swab/cms3.nsf/uploads/4F5A0D8E6F0DD139C1257E6E0051833A/$FILE/NethmapMaran2015%20_webversie.pdf

Catry, 2015, Use of colistin-containing products within the European Union and European Economic Area (EU/EEA): development of resistance in animals and possible impact on human and animal health., Int J Antimicrob Agents, 46, 297, 10.1016/j.ijantimicag.2015.06.005

Thông tin
Thông tin xuất bản

Eurosurveillance

Tập 21 Số 9

Thông tin tác giả