Preparative isolation and purification of three flavonoid glycosides from <i>Taraxacum mongolicum</i> by high‐speed counter‐current chromatography

Journal of Separation Science - Tập 31 Số 4 - Trang 683-688 - 2008
Shuyun Shi1, Yuping Zhang2, Yu Zhao3, Huang Ke-long2
1School of Chemistry and Chemical Engineering, Central South University, Changsha, China
2School of Chemistry and Chemical Engineering, Central South University, Changsha, China. Fax: +86-731-8879850
3College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China

Tóm tắt

AbstractA preparative high‐speed counter‐current chromatography (HSCCC) was successively applied to purify three flavonoid glycosides from the aerial part of Taraxacum mongolicum, a traditional Chinese medicine. Subsequent UV, MS, and NMR analyses have led to the characterization of three flavonoid glycosides including two new compounds isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐L‐arabinopyranoside and isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐D‐glucopyranoside, and a known compound, isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐D‐xyloypyranoside, which were first isolated from T. mongolicum. The two‐phase solvent system composed of ethyl acetate/n‐butanol/water (2:1:3, v/v/v) was performed in HSCCC. Consequently, a total of 25.7 mg isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐L‐arabinopyranoside, 19.1 mg isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐D‐glucopyranoside, and 10.6 mg isoetin‐7‐O‐β‐D‐glucopyranosyl‐2′‐O‐α‐D‐xyloypyranoside were obtained with purity of 98.7, 98.3, and 99.1%, respectively, as determined by HPLC from 500 mg enriched extract after cleaning‐up by polyamide resin.

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Thông tin xuất bản

Journal of Separation Science

Tập 31 Số 4

683-688

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