Preparation of lipase-surfactant complex for the catalysis of triglyceride hydrolysis in heterogeneous reaction systems

Bioprocess and Biosystems Engineering - Tập 15 - Trang 133-137 - 1996
Y. Isono1, H. Nabetani2, M. Nakajima2
1Central Research Institute, Dainichiseika Color & Chemicals Mfg. Co., Ltd., Tokyo, Japan
2Process Engineering Laboratory, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Ibaraki, Japan

Tóm tắt

To facilitate lipase (E.C.3.1.1.3) catalyzed hydrolysis reaction of triglycerides in heterogeneous reaction systems, lipase-surfactant complex (LSC) was prepared by mixing an aqueous solution of lipase and surfactant in ethanol. The LSC prepared from lipase MF-30 derived from Pseudomonas sp. and Nonion SP-60R (sorbitan mono-stearate) showed a high LSC yield and activity recovery. About 30% of the protein in the crude lipase was recovered in the LSC precipitate, and further addition of surfactant to the supernatant was ineffective. Our preparation method for the selective separation of the catalytic active protein from a crude enzyme was suggested.

Tài liệu tham khảo

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