Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves
Tóm tắt
Callistemon citrinus (Curtis.) (Family- Myrtaceae) is a popular evergreen shrub in Bangladesh. In the present study, the leaves of this plant have been assessed comprehensively for free radical scavenging, thrombolytic and membrane stabilizing activities. The leaves were collected, powdered and extracted with methanol. The extract was then concentrated and successively fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions. The extractives were investigated for free radical scavenging, thrombolytic and membrane stabilizing activities. In case of 1,1 diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide radical scavenging assays, the crude methanol extract of the leaves showed the highest free radical scavenging activity among the tested materials including standard ascorbic acid (p = 0.0000). Besides, this extract was also found significantly rich (p = 0.0000) in phenolics and flavonoids compared to other organic fractions. In thrombolytic study, the petroleum ether fraction exhibited significantly stronger thrombolysis (p = 0.024) than other leaf extractives but was weaker than the standard streptokinase. In membrane stabilizing assay, the activity of chloroform fraction was similar to that of standard acetylsalicylic acid (p = 1.000) in hypotonic solution induced hemolysis. However, membrane stabilization activity of this chloroform fraction was found significantly stronger than that of the standard (p = 0.0000) in heat induced hemolysis. This study has revealed the medicinal capabilities of different organic fractions of C. citrinus displaying free radical scavenging, thrombolysis and membrane stabilizing antiinflammatory potentials. Further bioactivity guided isolation is required to obtain pharmacologically secondary metabolites.
Tài liệu tham khảo
Harvey AL. Natural products in drug discovery. Drug Discov Today. 2008;13:894–901.
Veeresham C. Natural products derived from plants as a source of drugs. J Adv Pharm Technol Res. 2012;3:200–1.
Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001;109:69–75.
Fennell C, Lindsey K, McGaw L, Sparg S, Stafford G, Elgorashi E, Grace O, Van Staden J. Assessing African medicinal plants for efficacy and safety: pharmacological screening and toxicology. J Ethnopharmacol. 2004;94:205–17.
Hosseinzadeh S, Jafarikukhdan A, Hosseini A, Armand R. The application of medicinal plants in traditional and modern medicine: a review of Thymus vulgaris. Int J Clin Med. 2015;6:635–42.
Moura FA, de Andrade KQ, dos Santos JC, Goulart MO. Lipoic acid: its antioxidant and anti-inflammatory role and clinical applications. Curr Top Med Chem. 2015;15:458–83.
Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev. 2010;4:118–26.
Slevin M, Ahmed N, Wang Q, McDowell G, Badimon L. Unique vascular protective properties of natural products: supplements or future main-line drugs with significant anti-atherosclerotic potential? Vascular cell. 2012;4:9.
Chen C, Yang F-Q, Zhang Q, Wang F-Q, Hu Y-J, Xia Z-N. Natural products for antithrombosis. Evid Based Complement Alternat Med. 2015;2015:876426.
Scrivo R, Vasile M, Bartosiewicz I, Valesini G. Inflammation as “common soil” of the multifactorial diseases. Autoimmun Rev. 2011;10:369–74.
Fürst R, Zündorf I. Plant-derived anti-inflammatory compounds: hopes and disappointments regarding the translation of preclinical knowledge into clinical progress. Mediators Inflamm. 2014;2014:146832.
Shrestha S, Poudel A, Satyal P, Dosoky NS, Chhetri BK, Setzer WN. Chemical composition and biological activity of the leaf essential oil of Callistemon citrinus from Nepal. Am J Essent Oils Nat Prod. 2015;2:29–33.
Sutar N, Sutar R, Kumar M. Callistemon citrinus (bottle brush) an important medicinal plant: a review of its traditional uses, phytoconstituents and pharmacological properties. Ind Res J Pharm & Sci. 2014;1:68–77.
Netala S, Penmetsa R, Nakka S, Polisetty BL. Pharmacognostic study of Callistemon citrinus L. bark. Int J Pharm Pharm Sci. 2015;7:427–30.
Oyedeji O, Lawal O, Shode F, Oyedeji A. Chemical composition and antibacterial activity of the essential oils of callistemon citrinus and callistemon viminalis from South Africa. Molecules. 2009;14:1990.
Ali N, Ahmed G, Ali Shah S, Shah I, Ghias M, Khan I. Acute toxicity, brine shrimp cytotoxicity and relaxant activity of fruits of callistemon citrinus curtis. BMC Complement Altern Med. 2011;11:99.
Momin F, Kalai B, Patole N, Godse V, Shikalgar T, Naikwade N. Cardio protective activity of ethanolic extract of Callistemon lanceolatus leaves on doxorubicin-induced cardiomyopathy in rats. Bangladesh J Pharmacol. 2011;6:38–45.
Goyal PK, Jain R, Jain S, Sharma A. A Review on biological and phytochemical investigation of plant genus Callistimon. Asian Pac J Trop Biomed. 2012;2:S1906–1909.
VanWagenen BC, Larsen R, Cardellina JH, Randazzo D, Lidert ZC, Swithenbank C. Ulosantoin, a potent insecticide from the sponge Ulosa ruetzleri. J Org Chem. 1993;58:335–7.
Joshi A, Bhobe M, Sattarkar A. Phytochemical investigation of the roots of Grewia microcos Linn. J Chem Pharm Res. 2013;5:80–7.
Kchaou M, Salah HB, Mhiri R, Allouche N. Anti-oxidant and anti-acetylcholinesterase activities of Zygophyllum album. Bangladesh J Pharmacol. 2016;11:54–62.
Chaity FR, Khatun M, Rahman MS. In vitro membrane stabilizing, thrombolytic and antioxidant potentials of Drynaria quercifolia L., a remedial plant of the Garo tribal people of Bangladesh. BMC Complement Altern Med. 2016;16:184.
Gülçin İ, Huyut Z, Elmastaş M, Aboul-Enein HY. Radical scavenging and antioxidant activity of tannic acid. Arab J Chem. 2010;3:43–53.
Sikder MAA, Kaisar MA, Rashid MA, Millat MS, Sultana A. In vitro membrane stabilizing activity, total phenolic content, cytotoxic, thrombolytic and antimicrobial activities of Calliandra surinamensis (Wall.). J Pharmacog Phytochem. 2012;1:45–50.
Prasad S, Kashyap RS, Deopujari JY, Purohit HJ, Taori GM, Daginawala HF. Effect of Fagonia arabica (Dhamasa) on in vitro thrombolysis. BMC Complement Altern Med. 2007;7:36.
Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Membrane stabilizing activity - a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia. 1999;70:251–7.
Hoareau L, DaSilva EJ. Medicinal plants: a re-emerging health aid. Electron J Biotechnol. 1999;2:3–4.
Verma S, Singh S. Current and future status of herbal medicines. Vet World. 2008;1:347–50.
Tilburt JC, Kaptchuk TJ. Herbal medicine research and global health: an ethical analysis. Bull World Health Organ. 2008;86:594–9.
Tu Y. The discovery of artemisinin (qinghaosu) and gifts from Chinese medicine. Nature med. 2011;17:1217–20.
Schetter AJ, Heegaard NH, Harris CC. Inflammation and cancer: interweaving microRNA, free radical, cytokine and p53 pathways. Carcinogenesis. 2010;31:37–49.
Rice-Evans C. Flavonoid antioxidants. Curr Med Chem. 2001;8:797–807.
Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci. 1997;2:152–9.
Previtali E, Bucciarelli P, Passamonti SM, Martinelli I. Risk factors for venous and arterial thrombosis. Blood Transfus. 2011;9:120–38.
Hilleman D, Campbell J. Efficacy, safety, and cost of thrombolytic agents for the management of dysfunctional hemodialysis catheters: a systematic review. Pharmacotherapy. 2011;31:1031–40.
Tsikouris JP, Tsikouris AP. A review of available fibrin-specific thrombolytic agents used in acute myocardial infarction. Pharmacotherapy. 2001;21:207–17.
Chen C, Li SX, Wang SM, Liang SW. Investigation into the anti-thrombosis effect and contents of total saponins and flavonoids in the bioactive fraction of Naodesheng prescription. J Ethnopharmacol. 2012;144:208–12.
Pietri S, Maurelli E, Drieu K, Culcasi M. Cardioprotective and anti-oxidant effects of the terpenoid constituents of Ginkgo biloba extract (EGb 761). J Mol Cell Cardiol. 1997;29:733–42.
Saleem TK, Azeem AK, Dilip C, Sankar C, Prasanth NV, Duraisami R. Anti-inflammatory activity of the leaf extacts of Gendarussa vulgaris Nees. Asian Pac J Trop Biomed. 2011;1:147–9.
Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol. 2004;142:231–55.
Serafini M, Peluso I, Raguzzini A. Flavonoids as anti-inflammatory agents. Proc Nutr Soc. 2010;69:273–8.
Vijayalakshmi A, Ravichandiran V, Velraj M, Hemalatha S, Sudharani G, Jayakumari S. Anti-anaphylactic and anti-inflammatory activities of a bioactive alkaloid from the root bark of Plumeria acutifolia Poir. Asian Pac J Trop Biomed. 2011;1:401–5.