Potent activation of tumor necrosis factor-α production by a polysaccharide found in a marine brown alga Chordaria flagelliformis

Fisheries Science - Tập 78 - Trang 717-724 - 2012
Yoko Tokimura1, Uki Yamashita2, Yuto Kamei1
1Coastal Bioenvironment Center, Saga University, Karatsu, Japan
2Department of Immunology, School of Medicine, University of Occupational and Environmental Health, Yahatanishiku, Kitakyushu, Japan

Tóm tắt

In a search for new antitumor drugs, we collected 334 species of marine algae from the Japanese coastline and screened these for tumor necrosis factor-alpha (TNF-α) production-promoting activity in an in vitro assay with the mouse macrophage-derived cell line RAW264.7. A phosphate-buffered saline extract from the marine brown alga Chordaria flagelliformis was found to strongly promote TNF-α activity. Further purification of this alga by precipitation with ethanol and two-step chromatography yielded an approximately 3,000-kDa polysaccharide as the active substance, which we designated MC25. MC25 exhibited TNF-α production-promoting activity in RAW264.7 cells in a dose-dependent manner, and we established its temperature and pH stabilities and non-cytotoxic character. Its molecular structure is similar to that of a fucoidan in terms of composition, including glucose, fucose, xylose, and uronic acid. The molecule hydrolyzed to a size of 180–370 kDa still retained strong TNF-α production-promoting activity, suggesting that MC25 might be a promising candidate in the development of novel antitumor agents.

Tài liệu tham khảo

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