Potassium Transport Loci in Escherichia coli K-12

Journal of Bacteriology - Tập 108 Số 2 - Trang 639-644 - 1971
Wolfgang Epstein1, Byung‐Soo Kim1
1Department of Biochemistry, University of Chicago, Chicago, Illinois 60637

Tóm tắt

Mutants of Escherichia coli K-12 requiring considerably elevated concentrations of potassium for growth are readily obtained as double mutants combining a kdp mutation with a mutation in one or more of five other loci. These loci are referred to as trk , for transport of K, because these mutations result in alterations in K transport. The kdp mutation is essential in the isolation and identification of this type of mutant; in a Kdp + strain, the presence of a trk mutation does not prevent growth of the strain in media containing very low concentrations of K. The trk loci are widely scattered over the E. coli chromosome; none of them is very near any other trk locus or near the kdp genes.

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Tài liệu tham khảo

Burmeister , M. 1969 . Chromosomal location of a gene involved in potassium ion uptake in Escherichia coli B . J. Bacteriol. 100 : 796 - 802 .

Damadian , R. 1968 . Ion metabolism in a potassium accumulation mutant of Escherichia coli B. I. Potassium metabolism . J. Bacteriol. 95 : 113 - 122 .

Damadian , R. 1969 . Ion exchange in Escherichia coli: potassium-binding proteins . Science 165 : 79 - 81 .

Epstein , W. , and M. Davies . 1970 . Potassium-dependent mutants of Escherichia coli K-12 . J. Bacteriol. 101 : 836 - 843 .

Epstein , W. , and S. G. Schultz . 1965 . Cation transport in Escherichia coli. V. Regulation of cation content . J. Gen. Physiol. 49 : 221 - 234 .

Epstein , W. , and S. G. Schultz . 1966 . Cation transport in Escherichia coli. VI. K exchange . J. Gen. Physiol. 49 : 469 - 481 .

Gunther , T. , and F. Dorn . 1966 . Oiber die K-transport bei der K-Mangelmutante . Z. Naturforsch. Ser. B 21 : 1082 - 1088 .

Lubin , M. , and D. Kessel . 1960 . Preliminary mapping of the genetic locus for potassium transport in Escherichia coli . Biochem. Biophys. Res. Commun. 2 : 249 - 255 .

Lubochinsky , B. , J. Meury , and J. Stolkowski . 1964 . Cinetique des 6changes de potassium chez l'Escherichia coli, souche B 207, qui ne peut croitre normalement qu'en presence de concentrations 61ev 6es en potassium . C. R. Acad. Sci. Paris 258 : 5106 - 5109 .

Perlman , R. , G. Chen , B. deCrombrugghe , M. Emmer , M. Gottesman , H. Varmus , and I. Pastan . 1970 . The regulation of lac operon transcription by cyclic adenosine 3', 5'-monophosphate . Cold Spring Harbor Symp. Quant. Biol. 35 : 419 - 423 .

Perlman , R. L. , and I. Pastan . 1969 . Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coli . Biochem. Biophys. Res. Commun. 37 : 151 - 157 .

Pittard , J. , and T. Ramakrishnan . 1964 . Gene transfer by F' strains of Escherichia coli. IV. Effect of a chromosomal deletion on chromosome transfer . J. Bacteriol. 88 : 367 - 373 .

Schultz , S. G. , W. Epstein , and A. K. Solomon . 1963 . Cation transport in Escherichia coli. IV. Kinetics of net K uptake . J. Gen. Physiol. 47 : 329 - 346 .

Schultz , S. G. , and A. K. Solomon . 1961 . Cation transport in Escherichia coli. 1. Intracellular Na and K concentrations and net cation movement . J. Gen. Physiol. 45 : 355 - 369 .

Schultz S. G. N. L. Wilson and W. Epstein. Cation transport in Escherichia coli. 11. Intracellular chloride concentration. J. Gen. Physiol. 46:159-166.

Taylor , A. L. 1970 . Current linkage map of Escherichia coli . Bacteriol. Rev. 34 : 155 - 175 .

Taylor , A. L. , and C. D. Trotter . 1967 . Revised linkage map of Escherichia coli . Bacteriol. Rev. 31 : 332 - 353 .

Yokota , T. , and J. S. Gots . 1970 . Requirement of adenosine 3',5'-cylic phosphate for flagella formation in Escherichia coli and Salmonella typhimurium . J. Bacteriol. 103 : 513 - 516 .