Posttranslational Modifications of Tubulin and the Polarized Transport of Kinesin-1 in Neurons

Molecular Biology of the Cell - Tập 21 Số 4 - Trang 572-583 - 2010
Jennetta W. Hammond1, Chun-Fang Huang2, Stefanie Kaech2, Catherine Jacobson2,3, Gary Banker2, Kristen J. Verhey1
1*Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109; and
2Jungers Center for Neurosciences Research, Oregon Health and Science University, Portland, OR 97239
3University of California at San Francisco

Tóm tắt

Polarized transport by microtubule-based motors is critical for neuronal development and function. Selective translocation of the Kinesin-1 motor domain is the earliest known marker of axonal identity, occurring before morphological differentiation. Thus, Kinesin-1–mediated transport may contribute to axonal specification. We tested whether posttranslational modifications of tubulin influence the ability of Kinesin-1 motors to distinguish microtubule tracks during neuronal development. We detected no difference in microtubule stability between axons and minor neurites in polarized stage 3 hippocampal neurons. In contrast, microtubule modifications were enriched in a subset of neurites in unpolarized stage 2 cells and the developing axon in polarized stage 3 cells. This enrichment correlated with the selective accumulation of constitutively active Kinesin-1 motors. Increasing tubulin acetylation, without altering the levels of other tubulin modifications, did not alter the selectivity of Kinesin-1 accumulation in polarized cells. However, globally enhancing tubulin acetylation, detyrosination, and polyglutamylation by Taxol treatment or inhibition of glycogen synthase kinase 3β decreased the selectivity of Kinesin-1 translocation and led to the formation of multiple axons. Although microtubule acetylation enhances the motility of Kinesin-1, the preferential translocation of Kinesin-1 on axonal microtubules in polarized neuronal cells is not determined by acetylation alone but is probably specified by a combination of tubulin modifications.

Từ khóa


Tài liệu tham khảo

Arimura N., 2007, Nat. Rev. Neurosci, 8, 194, 10.1038/nrn2056

Baas P. W., 1989, J. Cell Biol, 109, 3085, 10.1083/jcb.109.6.3085

Baas P. W., 1991, J. Neurosci. Res, 30, 134, 10.1002/jnr.490300115

Barnes A. P., 2008, Curr. Opin. Neurobiol, 18, 44, 10.1016/j.conb.2008.05.003

Bielas S. L., 2007, Cell, 129, 579, 10.1016/j.cell.2007.03.023

Black M. M., 1987, J. Neurosci, 7, 3695, 10.1523/JNEUROSCI.07-11-03695.1987

Cai D., 2009, PLoS Biol, 7, e1000216, 10.1371/journal.pbio.1000216

Chang J., 2009, J. Cell Sci, 122, 2274, 10.1242/jcs.048975

Creppe C., 2009, Cell, 136, 551, 10.1016/j.cell.2008.11.043

Dajas-Bailador F., 2008, Curr. Biol, 18, 221, 10.1016/j.cub.2008.01.025

Dompierre J. P., 2007, J. Neurosci, 27, 3571, 10.1523/JNEUROSCI.0037-07.2007

Dotti C. G., 1987, Nature, 330, 254, 10.1038/330254a0

Dotti C. G., 1988, J. Neurosci, 8, 1454, 10.1523/JNEUROSCI.08-04-01454.1988

Dunn S., 2008, J. Cell Sci, 121, 1085, 10.1242/jcs.026492

10.1091/mbc.e05-10-0914

Gartner A., 2006, J. Cell Sci, 119, 3927, 10.1242/jcs.03159

Gomis-Ruth S., 2008, Curr. Biol, 18, 992, 10.1016/j.cub.2008.06.026

Goslin K., 1989, J. Cell Biol, 108, 1507, 10.1083/jcb.108.4.1507

Gurskaya N. G., 2006, Nat. Biotechnol, 24, 461, 10.1038/nbt1191

Haggarty S. J., 2003, Proc. Natl. Acad. Sci. USA, 100, 4389, 10.1073/pnas.0430973100

Hammond J. W., 2008, Curr. Opin. Cell Biol, 20, 71, 10.1016/j.ceb.2007.11.010

Hubbert C., 2002, Nature, 417, 455, 10.1038/417455a

Ikegami K., 2007, Proc. Natl. Acad. Sci. USA, 104, 3213, 10.1073/pnas.0611547104

Ikegami K., 2006, J. Biol. Chem, 281, 30707, 10.1074/jbc.M603984200

Jacobson C., 2006, Neuron, 49, 797, 10.1016/j.neuron.2006.02.005

Janke C., 2005, Science, 308, 1758, 10.1126/science.1113010

Jiang H., 2005, Cell, 120, 123

Jordan M. A., 1998, Methods Enzymol, 298, 252, 10.1016/S0076-6879(98)98024-7

Kaech S., 2006, Nat. Protoc, 1, 2406, 10.1038/nprot.2006.356

Kim W. Y., 2006, Neuron, 52, 981, 10.1016/j.neuron.2006.10.031

Konishi Y., 2009, Nat. Neurosci, 12, 559, 10.1038/nn.2314

Larcher J. C., 1996, J. Biol. Chem, 271, 22117, 10.1074/jbc.271.36.22117

Liao G., 1998, J. Biol. Chem, 273, 9797, 10.1074/jbc.273.16.9797

Matsuyama A., 2002, EMBO J, 21, 6820, 10.1093/emboj/cdf682

Meyer D., 1999, J. Biol. Chem, 274, 35113, 10.1074/jbc.274.49.35113

Nakata T., 2003, J. Cell Biol, 162, 1045, 10.1083/jcb.200302175

Ohkawa N., 2008, Genes Cells, 13, 1171, 10.1111/j.1365-2443.2008.01235.x

Owen R., 2003, Mol. Cell. Neurosci, 23, 626, 10.1016/S1044-7431(03)00095-2

Reed N. A., 2006, Curr. Biol, 16, 2166, 10.1016/j.cub.2006.09.014

Samsonov A., 2004, J. Cell Sci, 117, 6129, 10.1242/jcs.01531

Toriyama M., 2006, J. Cell Biol, 175, 147, 10.1083/jcb.200604160

van Dijk J., 2007, Mol. Cell, 26, 437, 10.1016/j.molcel.2007.04.012

Verhey K. J., 2007, Cell Cycle, 6, 2152, 10.4161/cc.6.17.4633

Verhey K. J., 2001, J. Cell Biol, 152, 959, 10.1083/jcb.152.5.959

Witte H., 2008, J. Cell Biol, 180, 619, 10.1083/jcb.200707042

Wolff A., 1992, Eur J. Cell Biol, 59, 425

Yoshimura T., 2005, Cell, 120, 137, 10.1016/j.cell.2004.11.012

Thông tin
Thông tin xuất bản

Molecular Biology of the Cell

Tập 21 Số 4

572-583

Thông tin tác giả