Polymorphic microsatellite loci for the trematode Diplostomum pseudospathaceum

Wiley - Tập 4 Số 4 - Trang 577-579 - 2004
Thorsten B. H. Reusch1, Gisep Rauch1, Martin Kalbe1
1Max Planck Institut für Limnologie, August-Thienemann Strasse 2, 24306 Plön, Germany

Tóm tắt

AbstractWe present primers for five polymorphic microsatellite loci in the eye fluke Diplostomum pseudospathaceum (Trematoda), a widely distributed parasite with a complex life cycle used as a model for parasitology and fish immunology. The loci were identified using a GA/CT‐enriched genomic library by subtractive hybridization with magnetic particles. All five loci were highly polymorphic, displaying 17 to 61 alleles and heterozygosities ranging from 0.53 to 0.92. We isolated populations of parasites within the first (snail) and second (fish) intermediate host and found small but significant genetic differentiation (FST = 0.012) between the two life stages of the parasite.

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Wiley

Tập 4 Số 4

577-579

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