Pollen allergen homologues in barley and other crop species

Clinical and Experimental Allergy - Tập 25 Số 1 - Trang 66-72 - 1995
James D. Astwood1, Shyam S. Mohapatra1, Heyu Ni2, Robert D. Hill1
1Departments of Plant Science, University of Manitoba, Winnipeg, Canada
2Departments of Immunology, University of Manitoba, Winnipeg, Canada

Tóm tắt

Summary

Pollen from 10 agricultural plant species was surveyed for the presence of proteins crossreactive with group I, group IV and group IX allergens. Barley (Hordeum vulgare), maize (Zea mays), rye (Secale cerale), triticale (xTriticosecale cereale), oats (Avena saliva), Canola (Brassica napus) and sunflower (Helianthus annux) pollens contained numerous allergen cognate proteins. Northern blot analysis of barley pollen RNA revealed the presence of group I and group IX allergen transcripts. The barley pollen cDNA hvp9742, and three other cloned allergens: phlenum protense (Phip) V, Phl p Va and Lolium perenne (Lol p) 1b, were demonstrated to have extensive nucleotide and amino acid sequence similarity to the Poa p IX isoallergens. It was concluded that hvp9742 represents a Poa p IX isoallergen homologue expressed by barley pollen, and was therefore designated Hor v IX. It is further shown that the most highly conserved domains of all seven proteins, including Hor v IX, map to previously defined Poa p IX antibody binding epitopes.

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Tài liệu tham khảo

10.1159/000236170

Silvanovich A., 1991, Nucleotide sequence analysis of three cDNAs coding for Poa p IX isoallergens of Kentucky bluegruss pollen, J Biol Chem, 266, 1204, 10.1016/S0021-9258(17)35302-4

10.1016/0161-5890(92)90175-W

10.1159/000236209

10.1159/000235530

Bassett IJ, 1978, An atlas of airborne pollen grains and common fungus spores of Canada

10.1159/000233901

Eriksson NE., 1984, Clustering of food‐stuffs in food hypersensi‐tivity, An inquiry study in pollen allergic patients. Alergol Immunopathol, 12, 28

Dominguez J, 1990, Purification and characterization of an allergen of mustard seed, Ann Allergy, 64, 352

10.1016/0014-5793(90)80642-V

10.1016/0014-5793(89)80444-2

10.1007/BF00019940

10.1159/000236171

10.1159/000234688

Jaggi KS, 1989, Identification of two distinct allergenic sites on ryegrass‐pollen allergen Lol p IV, J Clin Immunol, 83, 852

AstwoodJD HillRD.cDNAs homologous to pollen allergens encode putative cell adhesion/extracellular matrix receptor proteins.P1 Mol Biol(In Review).

10.1016/0003-2697(76)90527-3

10.1038/227680a0

10.1126/science.6162199

10.1073/pnas.76.9.4350

10.1021/bi00591a005

10.1073/pnas.69.6.1408

10.1073/pnas.74.12.5350

10.1016/0022-2836(77)90052-3

Sambrook J, 1989, Molecular cloning: a laboratory manual, second edition

10.1093/nar/10.20.6451

10.1073/pnas.85.8.2444

10.1016/0076-6879(90)83011-W

Deverequx J., 1980, The GCG Sequence analysis software package, version 6.0

Hatton TW, Allergy and Mol Biology, 174

10.1159/000235142

Vrtala S, 1993, cDNA cloning of a major allergen from timothy grass (Phleum pratense) pollen; characterization of the recombinant Phl p V allergen, J Immunol, 151, 4773, 10.4049/jimmunol.151.9.4773

BufeA BeckerW SchrammGet al.Major allergen Phl p Va (Timothy grass) bears at least two different IgE reactive epitopes.1993(unpublished‐Genbank accession number X70942).

10.1073/pnas.88.4.1384

10.1159/000234338

Astwood JD., 1990, Identification of developmentally regulated pollen specific mRNAs and proteins in barley, Plant Physiol, 102, 10

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Clinical and Experimental Allergy

Tập 25 Số 1

66-72

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