Pod and seed development and phenotype of the M1 plants after pollination and fertilization with irradiated pollen in cacao (Theobroma cacao L.)
Tóm tắt
In order to try to induce parthenogenesis, Theobroma cacao L. pollen of the clone K5 was gamma-irradiated at 0, 50, 70 and 90 Gy and used to pollinate flowers of the clones IMC67 and T85/799. Fruit survival rate 30 days after pollination decreased as pollen irradiation dose increased. LD50 was about 70 Gy for IMC67 and 75 Gy for T85/799. Pods obtained from irradiated pollen were smaller, ripened slower, and contained a lower number of beans than the controls. In many beans obtained after pollination with irradiated pollen, albumen was still present at maturity, and the embryo was either absent or abnormal and reduced in size, while control beans contained a well-developed embryo and almost no visible albumen. No haploid was obtained. Morphologic mutants were obtained from IMC67 pollinated with 50 Gy-irradiated K5 pollen, and some of them had inherited paternal alleles of enzymic markers without showing the axil-spot dominant character. Accidental self-fertilization of the two self-incompatible clones through mentor pollen effect, was observed in rare cases.
Tài liệu tham khảo
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