Platelet activation through interaction with hemodialysis membranes induces neutrophils to produce reactive oxygen species

Journal of Biomedical Materials Research - Part A - Tập 77A Số 2 - Trang 294-303 - 2006
Saotomo Itoh1, Chie Susuki1, Tsutomu Tsuji1
1Department of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan

Tóm tắt

AbstractThe intradialytic activation of leukocytes is one of the major causes of hemodialysis‐associated complications. During hemodialysis, the formation of microaggregates consisting of platelets and neutrophils has been observed to accompany the production of reactive oxygen species (ROS) by leukocytes. In this study, we investigated the interaction of platelets and neutrophils with hemodialysis membranes in vitro to elucidate the mechanism underlying microaggregate formation and its relevance to leukocyte activation. The production of ROS in neutrophils was induced by the coincubation of neutrophils with polysulfone (PS) membranes, and was increased when platelets were present in the neutrophil suspension. Neutrophils that were incubated with polymethylmethacrylate (PMMA) membranes in the presence of platelets also produced significant levels of ROS, suggesting that the presence of platelets augmented ROS production in neutrophils. Platelets adhered more firmly to hydrophobic membranes such as PS and PMMA membranes than to hydrophilic membranes, such as those composed of regenerated cellulose (RC) or ethylene vinylalcohol copolymer (EVAL). The adhesion of platelets to dialysis membranes composed of different materials was correlated with those membranes' ability to induce platelet activation as assessed by the cell surface expression of P‐selectin. Moreover, coincubation of neutrophils with platelets that had been treated with hydrophobic membranes induced a higher level of superoxide anion relative to those treated with hydrophilic membranes in association with the P‐selectin‐mediated microaggregate formation. These results suggest that platelets activated through interaction with hemodialysis membranes stimulate neutrophils to produce ROS via P‐selectin‐mediated adhesion, and that this property of adhesion to platelets is critical for the biocompatibility of hemodialysis membranes. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

Từ khóa


Tài liệu tham khảo

10.1038/ki.1993.272

Himmelfarb J, 1994, Biocompatibility and risk of infection in haemodialysis patients, Nephrol Dial Transplant, 9, 138

Hörl WH, 2002, Hemodialysis membranes: Interleukins, biocompatibility, and middle molecules, J Am Soc Nephrol, 13, S62

10.1515/CCLM.1999.058

10.1038/ki.1994.299

10.1161/01.ATV.20.12.2702

Nagata K, 1993, Activated platelets induce superoxide anion release by monocytes and neutrophils through P‐selectin (CD62), J Immunol, 151, 3267, 10.4049/jimmunol.151.6.3267

10.1111/j.1365-2141.1995.tb05197.x

10.1159/000189577

10.1177/039139889902200803

10.1177/039139880202500607

10.1083/jcb.101.3.880

10.1016/0952-7915(94)90037-X

Nagata K, 1994, Role of O‐linked carbohydrate chains on leukocyte cell membranes in platelet‐induced leukocyte activation, J Biol Chem, 269, 23290, 10.1016/S0021-9258(17)31652-6

10.1016/S0014-5793(00)01765-8

10.4049/jimmunol.130.4.1910

10.1016/0022-1759(81)90138-1

Spangenberg P, 1993, The platelet glycoprotein IIb/IIIa complex is involved in the adhesion of activated platelets to leukocytes, Thromb Haemost, 70, 514, 10.1055/s-0038-1649615

10.1093/intimm/12.4.487

10.1016/S0142-9612(01)00406-9

10.1053/ajkd.2000.8985

Siems W, 2002, Oxidative stress in chronic renal failure as a cardiovascular risk factor, Clin Nephrol, 58, S12

10.1161/01.CIR.101.9.1002

10.1016/S0946-672X(04)80031-2

10.1093/ndt/gfh569

10.1093/oxfordjournals.ndt.a091724

10.1007/978-1-4684-5718-6_4

Rosenkranz AR, 1999, Novel C5‐dependent mechanism of neutrophil stimulation by bioincompatible dialyzer membranes, J Am Soc Nephrol, 10, 128, 10.1681/ASN.V101128

Aljama P, 1978, Haemodialysis‐induced leucopenia and activation of complement: Effects of different membranes, Proc Eur Dial Transplant Assoc, 15, 144

10.1038/ki.1983.224

10.1038/ki.1984.155

10.1111/j.1525-1594.1997.tb00371.x

10.1159/000044903

10.1177/039139889601900705

10.1172/JCI117921

Elstad MR, 1995, P‐selectin regulates platelet‐activating factor synthesis and phagocytosis by monocytes, J Immunol, 155, 2109, 10.4049/jimmunol.155.4.2109

10.1073/pnas.91.19.8767

Thông tin
Thông tin xuất bản

Journal of Biomedical Materials Research - Part A

Tập 77A Số 2

294-303

Thông tin tác giả