Phytochemical Analysis and Biological Activities of the Ethanolic Extract of Daphne sericea Vahl Flowering Aerial Parts Collected in Central Italy

Biomolecules - Tập 11 Số 3 - Trang 379
Claudio Frezza1, Alessandro Venditti2, Daniela De Vita1, Fabio Sciubba2,3, Pierpaolo Tomai2, Marco Franceschin2, Mirella Di Cecco4, Giampiero Ciaschetti4, Antonella Di Sotto5, Annarita Stringaro6, Marisa Colone6, Alessandra Gentili2, Mauro Serafini1, Armandodoriano Bianco2
1Dipartimento di Biologia Ambientale, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185, Rome, Italy
2Dipartimento di Chimica, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185, Rome, Italy
3NMR-Based Metabolomics Laboratory, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, Italy
4Ufficio Monitoraggio e Conservazione della Biodiversità Vegetale, Parco Nazionale della Majella, Via Badia 28, 67039 Sulmona, Italy
5Dipartimento di Fisiologia e Farmacologia “V. Erspamer”, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, Italy
6Centro Nazionale per la Ricerca e la Valutazione Preclinica e Clinica dei Farmaci, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy

Tóm tắt

In this paper, the first phytochemical analysis of the ethanolic extract of Daphne sericea Vahl flowering aerial parts collected in Italy and its biological activities were reported. Eleven compounds were identified i.e., α-linolenic acid (1), tri-linoleoyl-sn-glycerol (2), pheophorbide a ethyl ester (3), pilloin (4), sinensetin (5), yuanhuanin (6), rutamontine (7), syringin (8), p-coumaric acid (9), p-anisic acid (10) and caffeic acid (11). To the best of our knowledge, compounds (1-4, 7-8 and 10) were isolated from D. sericea for the first time during this work, whereas sinensetin (5) represents a newly identified component of the entire Thymelaeaceae family. The extract was found to possess radical scavenging against both DPPH• and 2,2′-azino-bis(3-thylbenzothiazoline-6-sulfonic acid (ABTS•+) radicals, with at least a 40-fold higher potency against the latter. Moreover, chelating abilities against both ferrous and ferric ions have been highlighted, thus suggesting a possible indirect antioxidant power of the extract. Although the precise bioactive compounds remain to be discovered, the polyphenolic constituents, including phenolic acids, tannins and flavonoids, seem to contribute to the antioxidant power of the phytocomplex. In addition, the extract produced cytotoxic effects in MDA-MB-231 and U87-MG cancer cell lines, especially at the concentration of 625 μg/mL and after 48–72 h. Further studies are required to clarify the contribution of the identified compounds in the bioactivities of the extract and to support possible future applications.

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