Physically and chemically-crosslinked L-arginine-loaded polyvinyl alcohol- hyaluronic acid- cellulose nanocrystals hydrogel membranes for wound healing: influence of crosslinking methods on biological performance of membranes in-Vitro

Springer Science and Business Media LLC - Tập 9 Số 3 - Trang 304-316 - 2023
Yasmein Hussein1, Elbadawy A. Kamoun1, Samah A. Loutfy1, Rehab Amin2, Esmail M. El‐Fakharany3, Tarek H. Taha4, Medhat Amer5
1Nanotechnology Research Center (NTRC), The British University in Egypt (BUE), El-Sherouk City, Cairo, 11837, Egypt
2National Institute of Laser Enhanced Science (NILES), Cairo University, Giza 12613, Egypt
3Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), Alexandria, 21934, Egypt
4Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), Alexandria, 21934, Egypt
5Zoology Department, Faculty of Science, Cairo University, Cairo, 11796, Egypt

Tóm tắt

AbstractTwo types of polyvinyl alcohol-blended hyaluronic acid and cellulose nanocrystals (PVA/HA/CNCs) hydrogels membranes were developed as delivery agents for L-arginine using chemical/and/physical crosslinking methods for exploring biological performance of the obtained membranes. Chemically and physically/crosslinked membranes were fabricated by solution-casting and freeze-thawing techniques, respectively. The characterization of the crosslinked PVA/HA/CNCs/L-arginine membranes were conducted by SEM, FTIR, TGA, swelling ratio, andin—vitroL-arginine release. Furthermore, applying two types of crosslinked membranes in vitro were investigated in terms of cytotoxicity, hemocompatibility, and cell adhesion affinity ofHFB-4cell line. Chemical and physical-crosslinked membranes were verified through the esterification and hydrogen bonds interactions, respectively as shown the FTIR spectra. The chemically-crosslinked membranes showed more thermal and mechanical stability than the physically-crosslinked ones. Besides, the chemically-crosslinked hydrogels exhibited biphasic release behavior of L-arginine reached 50% in the first hour and 92% after 48 h. Notably, chemically-crosslinked membranes a somewhat affect the cell viability %, while both crosslinked membranes exhibited excellent cell adhesion affinity and low hemolysis. However, the physically- crosslinked members showed high cell viability ~ 172%, compared to the chemically-crosslinked membranes at 98.5%; and low hemolysisca. 0.84versus1.34 for chemically-crosslinked membranes. Accordingly, both types of crosslinked membranes have a potential for applying in biomedical applications, particularly for designing promising wound dressing biomaterials.

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