Phylogenetic relationships of seven palearctic members of the maculipennis complex inferred from ITS2 sequence analysis

Insect Molecular Biology - Tập 8 Số 4 - Trang 469-480 - 1999
M Marinucci1, Carlo Severini1, Pamela Mancini1, Marco Di Luca1
1Laboratory of Parasitology, Istituto Superiore di Sanita, Rome, Italy

Tóm tắt

AbstractThe sequences of the second internal transcribed spacer (ITS2) of ribosomal DNA (rDNA) were determined from seven palearctic mosquitoes species belonging to the Anopheles maculipennis species complex, namely An. atroparvus, An. labranchiae, An. maculipennis, An. messeae, An. melanoon, An. sacharovi and An. martinius. The length of the ITS2 ranged from 280 to 300 bp, with a GC content of 49.4–54.1%. With the exception of An. messeae, negligible levels of intraspecific polymorphism and no intrapopulation variation were observed. The phylogenetic relationships among the members of the maculipennis complex were inferred by maximum‐parsimony analysis of the paup program and the neighbour‐joining and maximun‐likelihood analysis of the phylip program. All of the trees obtained were almost identical in topology, although the relationships among three species, i.e. An. maculipennis, An. messeae and An. melanoon, remained unresolved. The phylogenies were in good agreement with the previous gene–enzyme and polytene chromosome banding pattern studies.

Từ khóa


Tài liệu tham khảo

Angelucci A.(1955)Tavole sinottiche sugli anofelini italiani. ACIS.Monografia degli Annali di Sanità Pubblica1.

Arnheim N., 1983, Evolution of Genes and Proteins, 38

Beckingham K., 1982, The Cell Nucleus, 205

10.4269/ajtmh.1995.53.478

Bullini L.A., 1982, Recent Developments in the Genetic of Insect Disease Vectors, 465

10.1038/295564a0

10.1111/j.1365-2583.1996.tb00034.x

10.1093/jmedent/33.1.109

Corradetti A., 1934, Ricerche sugli incroci tra le varietà di Anopheles maculipennis, Riv Malariol, 13, 707

10.2307/1308904

10.1038/299111a0

10.1038/290731a0

Falleroni D., 1926, Fauna anofelica italiana e suo ‘habitat’ (paludi, risaie, canali). Metodi di lotta contro la malaria, Riv Malariol, 5, 553

Felsenstein J.(1981)Phylogeny Inference Package ( phylip) Version 3.5.University of Washington Seattle.

10.1111/j.1558-5646.1985.tb00420.x

Fritz G.N., 1994, Sequence analysis of the ribosomal DNA internal transcribed spacer 2 from populations of Anopheles nuneztovari (Diptera, Culicidae), Mol Biol Evol, 11, 406

Fritz G.N., 1991, Diagnostic characterization of Anopheles freeborni and An. hermsi by hybrid crosses frequencies of polytene X chromosomes and rDNA restriction enzyme fragments, J Am Mosquito Control Assoc, 7, 198

10.1007/978-1-4684-4988-4_7

Hackett L.W., 1935, The varieties of Anopheles maculipennis and their relation to the distribution of malaria in Europe, Riv Malariol, 14, 45

10.1086/417338

10.1007/BF01731581

Kitzmiller J.B., 1967, Genetics of Insect Vectors of Disease, 151

10.1111/j.1365-2583.1997.tb00078.x

10.1111/j.1365-2915.1990.tb00453.x

10.1111/j.1365-2583.1994.tb00144.x

Porter C.H., 1991, Species‐diagnostic differences in a ribosomal DNA internal transcribed spacer from the sibling species Anopheles freeborni and Anopheles hermsi (Diptera, Culicidae), Am J Trop Med Hyg, 45, 271, 10.4269/ajtmh.1991.45.271

10.1093/genetics/119.4.943

10.1007/BF02603075

10.1111/j.1365-2583.1993.tb00076.x

Stegnii V.N., 1982, Recent Developments in the Genetic of Insect Disease Vectors, 454

Swofford D.L., 1993, paup : phylogenetic analysis using parsimony, Version 3.11

10.1016/0022-2836(87)90181-1

10.1093/nar/22.22.4673

White G.B.(1976)The place of morphological studies in the investigation of theAnophelesspecies complexes.World Health Organization VBC/SC/76.14.

White G.B., 1978, Systematic reappraisal of the Anopheles maculipennis complex, Mosquito Syst, 10, 13

10.1111/j.1365-2915.1997.tb00302.x

Thông tin
Thông tin xuất bản

Insect Molecular Biology

Tập 8 Số 4

469-480

Thông tin tác giả