Photochemical cross‐linking of plastically compressed collagen gel produces an optimal scaffold for corneal tissue engineering

Journal of Biomedical Materials Research - Part A - Tập 99A Số 1 - Trang 1-8 - 2011
Shengli Mi1, Vitaliy V. Khutoryanskiy2, Roanne R. Jones3,1, Xiuping Zhu4, Ian W. Hamley3, Che J. Connon1
1Stem Cells and Nanomaterials Laboratory, Reading School of Pharmacy, School of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6UB, United Kingdom
2Reading School of Pharmacy, School of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6UB, United Kingdom
3Chemistry Department, School of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6UB, United Kingdom
4Department of Ophthalmology, Shaanxi Institute of Ophthalmology, Xi'an 710002, People's Republic of China

Tóm tắt

AbstractThe experiments were designed to use photochemically cross‐linked plastically compressed collagen (PCPCC) gel to support corneal epithelial cells. A plastically compressed collagen (PCC) scaffold was photo cross‐linked by UVA in the presence of riboflavin to form a biomaterial with optimal mechanical properties. The breaking force, rheology, surgical suture strength, transparency, ultrastructure, and cell‐based biocompatibility were compared between PCPCC and PCC gels. The breaking force increased proportionally with an increased concentration of riboflavin. The stress required to reach breaking point of the PCPCC scaffolds was over two times higher compared to the stress necessary to break PCC scaffolds in the presence of 0.1% riboflavin. Rheology results indicated that the structural properties of PCC remain unaltered after UVA cross‐linking. The PCC gels were more easily broken than PCPCC gels when sutured on to bovine corneas. The optical density values of PCPCC and PCC showed no significant differences (p > 0.05). SEM analyses showed that the collagen fibres within the PCPCC gels were similar in morphology to PCC gels. No difference in cell‐based biocompatibility was seen between the PCPCC and PCC scaffolds in terms of their ability to support the ex vivo expansion of corneal epithelial cells or their subsequent differentiation evidenced by similar levels of cytokeratin 14. In conclusion, PCPCC scaffold is an optimal biomaterial for use in therapeutic tissue engineering of the cornea. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 1–8, 2011.

Từ khóa


Tài liệu tham khảo

10.1007/BF00357072

10.1002/mrd.20900

10.1097/00003226-200001000-00013

10.1007/s00417-005-0095-3

10.1007/s00441-005-0059-2

10.1167/iovs.05-1415

10.1371/journal.pone.0001147

10.1136/bjo.2008.153064

10.1089/ten.tea.2009.0711

10.1016/j.preteyeres.2004.04.002

10.1115/1.1688774

10.1002/jbm.a.32861

10.1016/S0002-9394(02)02220-1

10.1007/s00347-002-0700-3

10.1097/01.icu.0000233954.86723.25

10.1016/S0886-3350(03)00407-3

10.1007/s004179900111

10.1002/adfm.200500042

10.1021/bm1009238

10.5650/jos.58.437

10.1097/01.TP.0000110320.45678.30

10.1016/S0142-9612(96)00106-8

10.1016/S0142-9612(02)00166-7

10.1016/S0169-409X(01)00240-X

10.1111/j.1470-8744.1993.tb00229.x

10.3349/ymj.2001.42.2.172

10.1016/S0142-9612(98)00240-3

10.1002/1097-4636(200107)56:1<101::AID-JBM1074>3.0.CO;2-6

10.1002/jbm.820291108

Vollers SS, 2005, Determination of peptide oligomerization state using rapid photochemical crosslinking, Methods Mol Biol, 299, 11

Cho KS, 1999, Reactive oxygen species‐induced apoptosis and necrosis on bovine corneal endothelial cells, Invest Ophthalmol Vis Sci, 40, 911

Dollery C, 1991, Therapeutic Drugs, 2:24

10.1007/s003470050515

Kolozsvari L, 2002, UV absorbance of the human cornea in the 240‐ to 400‐nm range, Invest Ophthalmol Vis Sci, 43, 2165

Thông tin
Thông tin xuất bản

Journal of Biomedical Materials Research - Part A

Tập 99A Số 1

1-8

Thông tin tác giả