Phosphorylase isoenzymes of human brain
Tóm tắt
The isoenzyme pattern of phosphorylase in human brain was studied by polyacrylamide slab gel electrophoresis and activity was revealed by iodine stain and by autoradiography: A major fast-migrating band was similar to isoenzyme I of the heart; an intermediate band migrated like the hybrid cardiac isoenzyme II; and a slow-migrating band, best revealed by autoradiography, migrated like the “muscle” isoenzyme III. The radioactivity in bands II and III accounted for 42% of total activity in the heart and 37% in the brain. Antibodies against mature muscle phosphorylase inhibited 41% of enzyme activity in heart extracts and 25% in brain extracts. These studies show that the “muscle” isoenzyme is a component of brain phosphorylase and suggest that partial phosphorylase deficiency occurs in the brain of patients with McArdle disease.
Tài liệu tham khảo
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