Phosphatidylserine‐related adhesion of human erythrocytes to vascular endothelium

British Journal of Haematology - Tập 107 Số 2 - Trang 300-302 - 1999
Christèle Closse1, Jeanne Dachary-Prigent2, M Boisseau3
1Laboratoire d'Hématologie, Université Victor Ségalen-Bordeaux 2, Bordeaux, France.
2UMR 5533 CNRS, Hôpital Cardiologique Haut Lévèque, Pessac, France
3Laboratoire d'Hématologie, Université Victor Ségalen Bordeaux 2, Bordeaux

Tóm tắt

In pathological conditions such as sickle cell disease, falciparum malaria and diabetes, an abnormal adherence of erythrocytes to endothelium is concomitant with loss of phospholipid asymmetry resulting in phosphatidylserine (PS) exposure. We have investigated the involvement of PS in this interaction by studying adhesion of human erythrocytes, treated with Ca2+‐ionophore A23187 in combination with N‐ethylmaleimide, to human umbilical vein endothelial cells in a flow‐based assay. Results showed that erythrocytes which exposed PS, massively adhered to HUVEC in a Ca2+‐dependent manner. This adhesion was inhibited by PS liposomes and by annexin V, giving clear evidence of the PS dependence of these interactions.

Từ khóa


Tài liệu tham khảo

Cines D.B., 1998, Endothelial cells in physiology and in the pathophysiology of vascular disorders, Blood, 91, 3527

10.1006/mvre.1993.1004

10.1182/blood.V81.10.2554.2554

10.1038/sj.cdd.4400404

10.1172/JCI107470

10.1182/blood.V87.3.1179.bloodjournal8731179

10.1006/jmbi.1994.1129

10.1042/bj3170343

10.1002/jcp.1041230210

Sulpice J.C., 1994, Requirement for phosphatidylinositol‐4,5‐biphosphate in the calcium‐induced phospholipid redistribution in the human erythrocyte membrane, Journal of Biological Chemistry, 269, 6347, 10.1016/S0021-9258(17)37378-7

Thevenin B.J.‐M., 1997, Band 3 peptides block the adherence of sickle cells to endothelial cells in vitro, Blood, 90, 4172, 10.1182/blood.V90.10.4172

Wali R.K., 1988, Alterations in organization of phospholipids in erythrocytes as factor in adherence to endothelial cells in diabetes mellitus, Diabetes, 37, 104, 10.2337/diab.37.1.104

Thông tin
Thông tin xuất bản

British Journal of Haematology

Tập 107 Số 2

300-302

Thông tin tác giả