Phenotypic Analysis of Antigen-Specific T Lymphocytes

American Association for the Advancement of Science (AAAS) - Tập 274 Số 5284 - Trang 94-96 - 1996
John D. Altman1, Paul Moss2, Philip Goulder2, Dan H. Barouch2, Michael G. McHeyzer‐Williams1, John I. Bell2, Andrew J. McMichael2, Mark M. Davis3
1J. D. Altman and M. G. McHeyzer-Williams, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5428, USA.
2P. A. H. Moss, P. J. R. Goulder, D. H. Barouch, J. I. Bell, A. J. McMichael, Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK.
3M. M. Davis, The Howard Hughes Medical Institute, Department of Microbiology and Immunology, Beckman Center, Room B221, Stanford University, Stanford, CA 94305-5428, USA.

Tóm tắt

Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens.

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American Association for the Advancement of Science (AAAS)

Tập 274 Số 5284

94-96

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