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Tác động của tiền điều kiện hóa dược lý: Prostaglandin E1 kích thích protein sốc nhiệt ngay sau khi thiếu máu/tái tưới máu ở gan chuột
Tóm tắt
Prostaglandin E1 (PGE1) có nhiều tác dụng điều trị tiềm năng, bao gồm bảo vệ tế bào, giãn mạch và ức chế sự kết tụ tiểu cầu. Nghiên cứu này điều tra tác động bảo vệ của PGE1 chống lại tổn thương do thiếu máu/tái tưới máu ở gan trong điều kiện in vivo bằng cách sử dụng microarray DNA bổ sung. PGE1 hoặc dung dịch muối sinh lý được truyền tĩnh mạch liên tục cho chuột, trong đó thùy trái của gan bị làm thiếu máu trong 30 phút và sau đó được tái tưới máu. Gan được thu hoạch sau 0, 10 và 30 phút sau khi tái tưới máu. RNA thông tin được tách ra, và các mẫu được gán nhãn bằng hai màu huỳnh quang khác nhau và lai ghép với bộ microarray DNA bổ sung chuột dài đầy đủ RIKEN với 18,816 gen. Mức alanine aminotransferase huyết thanh và aspartate aminotransferase ở 180 phút sau tái tưới máu thấp đáng kể ở nhóm được điều trị bằng PGE1 so với nhóm được điều trị bằng dung dịch muối sinh lý. Phân tích microarray cDNA cho thấy rằng các gen mã hóa protein sốc nhiệt (HSP) 70, protein điều hòa glucose 78, HSP86 và glutathione S-transferase đã được tăng cường trong giai đoạn cuối của thời gian thiếu máu (0 phút sau tái tưới máu) trong nhóm PGE1. Kết quả của chúng tôi cho thấy rằng PGE1 kích thích HSP ngay lập tức sau khi thiếu máu tái tưới máu. HSP do đó có thể đóng một vai trò quan trọng trong tác dụng bảo vệ của PGE1 chống lại tổn thương do thiếu máu/tái tưới máu ở gan.
Từ khóa
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