Pharmacokinetics and anesthetic activity of eugenol in male Sprague–Dawley rats

Journal of Veterinary Pharmacology and Therapeutics - Tập 29 Số 4 - Trang 265-270 - 2006
Sarah Annie Guénette1, Francis Beaudry1, Jean‐François Marier1, Pascal Vachon1
1Department of Veterinary Biomedicine, Faculty of Veterinary Medicine, University of Montreal, St Hyacinthe, Canada

Tóm tắt

Eugenol, the principle chemical constituent of clove oil, has recently been evaluated for its anesthetic and analgesic properties in fish and amphibians. The objective of this study was to determine the pharmacokinetic (PK) and anesthetic activity of eugenol in rats. Male Sprague–Dawley rats received single i.v. doses of eugenol (0, 5, 10, 20, 40 and 60 mg/kg) and anesthetic level was evaluated with the withdrawal reflex. For the 20 mg/kg dose level, blood and urinary samples were collected over 1 h for the PK assessment. Plasma and blood concentrations of eugenol, as well as metabolite identification in urine, were determined using a novel dansyl chloride derivatization method with liquid chromatography mass spectrometry (LC/MS/MS). PK parameters were calculated using noncompartmental methods. Eugenol‐induced loss of consciousness in a dose‐dependent manner, with mean (±SEM) recovery in reflex time of 167 ± 42 sec observed at the highest dose level. Mean systemic clearance (Cl) in plasma and blood were 157 and 204 mL/min/kg, respectively. Glucuronide and sulfate conjugates were identified in urine. Overall, eugenol produced a reversible, dose‐dependent anesthesia in male Sprague–Dawley rats.

Từ khóa


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Thông tin
Thông tin xuất bản

Journal of Veterinary Pharmacology and Therapeutics

Tập 29 Số 4

265-270

Thông tin tác giả