Pertussis toxin‐dependent and ‐independent hormonal effects on cultured renal epithelioid cells

FEBS Letters - Tập 234 Số 2 - Trang 263-266 - 1988
Markus Paulmichl1, Ewald Wöll, Florian Läng
1Institute for Physiology, University of Innsbruck, Austria.

Tóm tắt

The present study has been performed to test for the involvement of pertussis toxin‐sensitive GTP‐binding proteins (G‐proteins) in the cellular transduction of hormone‐induced activation of potassium channels. In Madin Darby canine kidney (MDCK) cells, a permanent cell line from dog kidney, epinephrine, acetylcholine, bradykinin, serotonin and ATP hyperpolarize the cell membrane by activation of potassium channels. In cells pretreated with pertussis toxin the hyperpolarizations elicited by either acetylcholine or serotonin are completely abolished; that following epinephrine is blunted and only transient. The hyperpolarizing effects of ATP or bradykinin are not affected by pertussis toxin. Thus, in MDCK cells both pertussis toxin‐dependent and ‐independent mechanisms operate in parallel to enhance the potassium conductance of the cell membrane.

Từ khóa


Tài liệu tham khảo

10.1113/jphysiol.1984.sp015195

10.1073/pnas.81.2.611

10.1152/physrev.1987.67.3.1054

10.1016/0143-4160(83)90020-9

10.1152/physrev.1987.67.4.1143

Simmons N.L., 1984, Fed. Proc., 43, 2225

10.1007/BF00590938

Lang F., 1988, Biochim. Biophys. Acta

10.1007/BF00581137

10.1007/BF00587718

Jungwirth A., 1988, Pflügers Arch., 411

10.1073/pnas.79.10.3129

10.1007/BF00580668

Lynch C.J., 1986, Mol. Pharmacol., 29, 196

Terman B.I., 1987, Mol. Pharmacol., 31, 12

10.1038/319670a0

10.1016/0014-2999(87)90157-9

10.1016/S0021-9258(17)44366-3

10.1016/0014-2999(87)90224-X

10.1073/pnas.84.18.6374

10.1042/bj2440129

10.1016/0006-291X(87)91126-0

10.1073/pnas.83.19.7320

10.1016/S0021-9258(17)39597-2

10.1111/j.1471-4159.1987.tb05712.x

10.1073/pnas.83.4.942

10.1016/0014-5793(86)80263-0

10.1016/0014-5793(87)81552-1

10.1016/0169-328X(87)90004-0

10.1038/325259a0

10.1038/317536a0

10.1007/BF00585301

10.1038/325321a0

10.1126/science.2432660

10.1073/pnas.84.12.4313

10.1007/BF00580674

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FEBS Letters

Tập 234 Số 2

263-266

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