Pathological and microbiological findings from incidents of unusual mortality of the common frog ( <i>Rana temporaria</i> )

Philosophical Transactions of the Royal Society B: Biological Sciences - Tập 351 Số 1347 - Trang 1539-1557 - 1996
A.A. Cunningham1, Tom Langton, Peter M. Bennett, Jackie Lewin, S. Drury, R. Gough, S. K. Macgregor
1Veterinary Science Group, Institute of Zoology, London, U.K.

Tóm tắt

In 1992 we began an investigation into incidents of unusual and mass mortalities of the common frog ( Rana temporaria ) in Britain which were being reported unsolicited to us in increasing numbers by members of the public. Investigations conducted at ten sites of unusual mortality resulted in two main disease syndromes being found: one characterized by skin ulceration and one characterized by systemic haemorrhages. However, frogs also were found with lesions common to both of these syndromes and microscopic skin lesions common to both syndromes were seen. The bacterium Aeromonas hydrophila, which has been described previously as causing similar lesions, was isolated significantly more frequently from haemorrhagic frogs than from those with skin ulceration only. However, as many of the latter were euthanased, this may have been due to differences in post mortem bacterial invasion. An iridovirus-like particle has been identified on electron microscopical examination of skin lesions from frogs with each syndrome and iridovirus-like inclusions have been detected in the livers of frogs with systemic haemorrhages. Also, an adenovirus-like particle has been cultured from one haemorrhagic frog. A poxvirus-like particle described previously from diseased frogs has now been found also in control animals and has been identified as a melanosome. Both the prevalence of the iridovirus-like particle and its association with lesions indicate that it may be implicated in the aetiology of the disease syndromes observed. Specifically, we hypothesize that primary iridovirus infection, with or without secondary infection with opportunistic pathogens such as A. hydrophila , may cause natural outbreaks of ‘red-leg’, a disease considered previously to be due to bacterial infection only.

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Tài liệu tham khảo

Alves de Matos A. P., 1993, Ultrastructure of erythrocytic virus of the South African anuran Ptychadena anchietae. Dis. aquat, Org, 16, 105

Anver M. R. & Pond C. L. 1984 Biology and Diseases of Amphibians. In Laboratory animal medicine (ed. J. G. Fox B. J. Cohen & F. M. Loew) pp. 427-447. Orlando: Academic Press.

10.2307/1564645

Briggs R. T., 1973, Fine structure of an amphibian leukocyte virus, J. Submicr. Cytol, 5, 71

10.1046/j.1523-1739.1993.07020355.x

10.1016/0042-6822(73)90438-8

Cullen B. R. Owens L. & Whittington R. J. Experimental infection of Australian 1995

anurans (Limnodynastes terraereginae and Litoria latopalmata) with Bohle iridovirus. Dis. aquat. Org 23 83-92.

10.1136/vr.133.6.141

10.1136/vr.137.3.72

10.1084/jem.7.1.32

Fawcett D. W. 1981 The Cell 2nd edn. pp. 537-550. Philadelphia London Toronto: W. B. Saunders Company.

Fijan N. 1988 Fish Vaccination (ed. A. E. Ellis) p. 204. London: Academic Press.

Fijan N. Matasin Z. Petrinec Z. Valpotic I. & Zwillenberg L. O. 1991 Isolation of an iridovirus-like agent from the green frog (Rana esculenta L.). Vet. arhiv. 61 151-158.

10.1111/j.1469-7998.1974.tb03153.x

Fox H., 1977, The anuran tadpole skin: changes occurring in it during metamorphosis and some comparisons with that of the adult, Symp. Zool. Soc. Lond, 39, 269

Ghadially F. N. 1980 Diagnostic Electron Microscopy of Tumours pp.78-87. London Boston: Butterworths.

Gibbs E. L., 1966, Rana pipiens: Health and disease, Lab. Anim. Care, 16, 142

Glorioso J. C., 1974, Microbiological studies on septicaemic bullfrogs (Rana catesbeiana), Am. J. Vet. Res, 35, 1241

Goorah R. M. & Granoff A. 1994 Encyclopaedia of Virology. Volume 1 (ed. R. G. Webster & A. Granoff) p. 503. London & San Diego: Academic Press.

Granoff A. 1989 Viruses ofLower Vertebrates (ed. W. Ahne & E. Kurstak) p. 3. Berlin: Springer-Verlag.

Hird D. W., 1981, Aeromonas hydrophila in wild-caught frogs and tadpoles (Rana pipiens) in Minnesota, Lab. Anim. Sci, 31, 166

Kirkwood B. R. 1988 The chi-squared test for contingency tables. In Essentials of medical statistics pp. 87-93. Oxford: Blackwell Scientific Publications.

Kunst Lj. & Valpotic I. 1968 Nova zarazna bolest zaba uzrokovana virusom. Vet. arhi 38 108-113.

10.1111/j.1365-2761.1987.tb01073.x

10.1111/j.1365-2761.1988.tb00527.x

Laurance W. F., 1995, Is a virus decimating frog populations, Aliens. Newsletter ofthe Invasive Species Specialist Group of the IUCN Species Survival Commissio, 1, 7

10.1136/gut.36.4.564

Mi§calencu D., 1981, Viral particles in the hepatocytes of Rana esculenta (L.), Rev. Roum. Med.-Virol, 32, 123

10.2307/1563944

Reichenbach-Klinke H. & Elkan E. 1965 The principal diseases of lower vertebrates. Book II Diseases of amphibians pp. 233-235. Hong Kong: T. F. H. Publications Inc.

10.1001/jama.1898.72440770010001d

Sanarelli G., 1891, Ueber einen neuen Mikroorganismus des Wassers, welcher fur Thiere mit veranderlicher und konstanter Temperatur pathogen ist, Zentr. Bakt. Parasitenk, 9, 193

van der Waaij D., 1974, Colonization patterns of aerobic Gram-negative bacteria in the cloaca of Rana pipiens, Lab. Anim. Sci, 24, 307

10.1093/infdis/118.3.253

10.3181/00379727-145-37981

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Philosophical Transactions of the Royal Society B: Biological Sciences

Tập 351 Số 1347

1539-1557

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