Hanako Shimizu1, Akihiko Sekizawa1, Yuditiya Purwosunu1,2, Masamitsu Nakamura1, Antonio Farina3, Nicola Rizzo3, Takashi Okai1
1Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan
2Department of Obstetrics and Gynecology, University of Indonesia, Jakarta, Indonesia
3Division of Prenatal Medicine, Department of Histology and Embryology, University of Bologna, Bologna, Italy
Tóm tắt
AbstractObjectiveTo assess the PP13 expression in the cellular component of blood in both preeclamptic patients and asymptomatic pregnant women during the early second trimester.MethodsIn the case–control study, peripheral blood samples were obtained from pregnant women with preeclampsia (n = 24) and controls (n = 22). PP13 RNA expression was quantified in the cellular component of the blood by reverse transcription PCR assay. Next, as a cohort study of asymptomatic pregnant women at early gestation, cellular RNA from 41 cases who developed preeclampsia at later gestation and 123 cases of control were analysed, and the possibility of prediction of preeclampsia was assessed.ResultsIn symptomatic patients, PP13 level in preeclampsia was significantly lower than that in controls (p < 0.001). In the asymptomatic pregnant women, the PP13 level in preeclampsia was significantly lower than that in the controls (p = 0.008). A receiver‐operating characteristics (ROC) curve yielded a detection rate of 24 and 31% at a false positive rate of 5 and 10% respectively.ConclusionAn alteration in the cellular PP13 RNA expression would affect the placental pathophysiological change. The cellular PP13 expression level could therefore potentially be one of the key markers to predict the clinical onset of preeclampsia. Copyright © 2009 John Wiley & Sons, Ltd.
